人工仿生脊髓导管的制备及性能分析  

Preparation and performance of a bionic spinal catheter

作　　者：朱祥[1] 陈旭义[2] 李瑞欣[3] 邢冉[1] 李东[4] 涂悦[2] 

机构地区：[1]天津中医药大学,天津市300193 [2]武警后勤学院附属医院脑科医院,脑创伤与神经疾病研究所,天津市神经创伤修复重点实验室,天津市300162 [3]解放军军事医学科学院卫生装备研究所,天津市300161 [4]天津医科大学总医院骨科,天津市300052

出　　处：《中国组织工程研究》2016年第21期3045-3050,共6页Chinese Journal of Tissue Engineering Research

基　　金：天津市科技支撑计划重点项目(14ZCZDGX00500);国家自然科学基金青年项目(11102235);天津市自然科学基金重点项目(12JCZDJC24100);天津市卫生局科技基金项目(2014KZ135);武警后勤学院附属医院种子基金项目(FYM201432)~~

摘　　要：背景:传统的组织工程导管制造方法存在复杂外形制造困难和内部空间结构无法控制的缺陷,不能满足一些微导管所要求的精确性和空间结构复杂性。目的:设计一种人工仿生脊髓导管,并研究其性能。方法:采用Solid Works制图软件建立导管数据模型,在3D打印机上生成平台扫描路径,以丝素蛋白和胶原蛋白为原料,制作人工仿生脊髓导管,检测导管的吸水率、孔隙率、力学性能及细胞相容性。将人工仿生脊髓导管置入SD大鼠背部皮下,置入后1,2,3,4周取出导管,观察导管降解情况。结果与结论:人工仿生脊髓导管的微观孔隙分布均匀,大小不等,孔径为10-240μm,孔隙率为(53.6±1.0)%,吸水膨胀率为(1 347.0±19.4)%,压缩弹性模量为(0.60±0.12)MPa;神经干细胞可在人工仿生脊髓导管孔隙中生长,呈球形或梭形,在导管表面密集生长,有些细胞伸出伪足附着在导管表面。人工仿生脊髓导管置入SD大鼠背部皮下1周后降解20%,2周后降解59%,3周时降解74%,4周后完全降解。结果表明人工仿生脊髓导管具有良好的细胞相容性及降解性。BACKGROUND： The traditional method of preparing tissue-engineered conduit has the defects of complex shape manufacturing and uncontrollable inner space structure, which cannot meet the requirements of some micro-catheters. OBJECTIVE： To prepare a bionic spinal catheter and analyze its performance. METHODS： The data model of the conduit was established using Solid Works software, and platform scan path was generated on three-dimensional printer to produce the bionic spinal catheter with fibroin and collagen as raw materials. Then the water absorption, porosity, mechanical properties and cellular compatibility of the conduits were detected. Next, the conduits were implanted into the subcutaneous tissue of rats and taken out at 1, 2, 3 and 4 weeks after surgery, respectively, to observe the degradation. RESULTS AND CONCLUSION： The porosity of the conduit was（53.6±1.0）%, the water absorption was（1347±19.4）%, and the compression modulus was（0.60±0.12） MPa. The micropores distributed uniformly with different size ranging from 10 to 240 μm. Spherical or fusiform stem cells survived in the pores and densely adhered to the conduit with pseudopodia. The degradation rate of the conduit was 20%, 59%, 74% and 100% at 1, 2, 3 and 4 weeks after surgery, respectively. These findings indicate that the artificial bionic spinal catheter has good biocompatibility and degradability.

关 键 词：生物降解 环境 脊髓 组织工程 生物材料 材料相容性 丝素蛋白 胶原蛋白 3D打印 人工仿生脊髓导管 国家自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]