万古霉素/羟基磷灰石/钛金属纳米管的生物相容性  

Biocompatibility of vancomycin/hydroxyapatite/titanium nanotubes

作　　者：张杭州[1] 田昂[2] 梁庆威[1] 白希壮[3] 薛向欣[2] 

机构地区：[1]中国医科大学附属第一医院运动医学科/关节外科,辽宁省沈阳市110001 [2]东北大学材料与冶金学院,辽宁省沈阳市110016 [3]中国医科大学附属人民医院运动医学科/关节外科,辽宁省沈阳市110006

出　　处：《中国组织工程研究》2016年第21期3097-3103,共7页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金(81071449;81501857);辽宁省省直医院改革重点临床科室诊疗能力建设项目(LNCCC-A03-2014)~~

摘　　要：背景:为了克服单一材料的缺点,羟基磷灰石/金属基复合材料同时负载抗生素的研究已引起人们的关注。目的:检测万古霉素/羟基磷灰石/钛金属纳米管的生物相容性。方法:将商业钛金属、二氧化钛纳米管及万古霉素/羟基磷灰石/钛金属纳米管分别与小鼠成骨细胞系MC-3T3-E1共培养,培养1,3,5 d时,倒置显微镜、扫描电镜下观察细胞生长情况;培养1,3,5 d时,AO-EB法检测细胞增殖;培养7,14 d时,检测细胞内总蛋白、钙与碱性磷酸酶水平。结果与结论:与商业钛金属组、二氧化钛纳米管组比较,万古霉素/羟基磷灰石/钛金属纳米管组小鼠成骨细胞MC-3T3-E1黏附良好,细胞活性及细胞形态良好,细胞生出大量伪足黏附于复合物表面;万古霉素/羟基磷灰石/钛金属纳米管组细胞数量多于商业钛金属组、二氧化钛纳米管组,细胞内钙、碱性磷酸酶水平高于商业钛金属组、二氧化钛纳米管组。结果表明,万古霉素/羟基磷灰石/钛金属纳米管具有良好的生物相容性,无生物毒性。BACKGROUND： In order to overcome the shortcomings of single materials, antibiotics-loaded hydroxyapatite/titanium composites have attracted people＇s attentions. OBJECTIVE： To evaluate the biocompatibility of vancomycin/hydroxyapatite/titanium nanotubes. METHODS： Mouse osteoblasts, MC-3T3-E1, were co-cultured with titanium（Cp-T）, TiO 2 nanotubes, and vancomycin/hydroxyapatite/titanium nanotubes, respectively. Cell morphology and growth were observed after 1, 3 and 5 days of co-culture under inverted microscope and scanning electron microscope. The cell proliferation was detected by AO-EB method. The total protein, calcium and alkaline phosphatase levels were detected at 7 and 14 days of co-culture. RESULTS AND CONCLUSION： The MC-3T3-E1 cells with good viability and morphology adhered well on the surface of vancomycin/hydroxyapatite/titanium nanotubes compared with those on the surface of pure titanium and TiO 2 nanotubes under the scanning electron miscroscope. Moreover, there were a large amount of pseudopodia on the surface of composite nanotubes. Compared with the other two groups, the cell number on the surface and the levels of intracellular calcium and alkaline phosphatase were all higher in the vancomycin/hydroxyapatite/titanium nanotubes group. These findings suggest that the vancomycin/hydroxyapatite/titanium nanotubes have good biocompatibility and no cytotoxicity.

关 键 词：万古霉素 硬羟基磷灰石 纳米管 组织工程 生物材料 材料相容性 羟基磷灰石 钛纳米管 成骨细胞 国家自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]