猪小肠黏膜下基质海绵的制备  

Preparation of the small intestinal submucosa sponge

作　　者：孙慧哲[1] 田伟[1] 曾亮[1] 王效杰[1] 王正东[1] 任玥[1] 匡宝平[1] 

机构地区：[1]沈阳医学院解剖教研室,辽宁省沈阳市110034

出　　处：《中国组织工程研究》2016年第21期3110-3116,共7页Chinese Journal of Tissue Engineering Research

基　　金：2014年省教育厅科学研究一般项目(L2014416)~~

摘　　要：背景:研究发现将小肠黏膜下基质直接应用于损伤部位,对体内外细胞的生长、分化还不是非常有效,而且其孔径很小,通透性不良,治疗效果不够理想。目的:构建小肠黏膜下基质海绵,了解其形态学特征。方法:采用物理及顺序化学浸泡脱细胞法制备猪小肠黏膜下基质。以不同质量分数的小肠黏膜下基质(1%、2%、3%、4%),不同浓度的交联剂EDC(50,100,150 mmol/L)制备小肠黏膜下基质海绵,光学显微镜及扫描电镜观察海绵结构。将小肠黏膜下基质海绵(实验组)与小肠黏膜下基质(对照组)分别置入大鼠背部肌肉中,置入1,2,3周后组织学观察组织反应及材料降解。结果与结论:(1)以质量分数1%小肠黏膜下基质、100 mmol/L EDC交联制得的小肠黏膜下基质海绵,空间结构弹性好,结构规整,孔径均匀一致,无空洞现象,所以肌肉置入实验选择此组材料;(2)置入1周后,实验组海绵网孔状结构保持完整,在材料周围可见轻微炎细胞浸润,少量嗜中性粒细胞、淋巴细胞浸润和巨细胞反应,支架边缘可见周围软组织增殖移行;对照组炎细胞浸润明显,其下创面有粘连,周围软组织增殖移行较少。置入3周后,实验组置入部位炎症基本消退,可见成纤维样细胞及血管成分,胶原纤维束较细小,平行排列,海绵处呈结缔组织样结构;对照组仍然存在炎性细胞浸润,胶原纤维含量较少;(3)结果表明:小肠黏膜下基质海绵结构合理,具备作为组织工程皮肤支架的潜能。BACKGROUND： Studies have found that small intestinal submucosa that is directly implanted into the lesion cannot effectively promote cell growth and differentiation in vivo and in vitro, because of its small pore size and poor permeability. OBJECTIVE： To establish the small intestinal submucosa sponge and to explore its morphological characteristics. METHODS： Porcine small intestinal submucosa was prepared by physiochemical method. Then the small intestinal submucosa with the mass fraction of 1%, 2%, 3% and 4% was cross-linked by 50, 100 and 150 mmol/L 1-ehyl-3-（3-dimethylaminopropyl） carbodiimide hydrochloride, respectively, so as to obtain small intestinal submucosa sponge, whose morphology was detected by lighting and scanning electron microscope. In the meanwhile, small intestinal submucosa as control group, and small intestinal submucosa sponge as test group were intramuscularly implanted into the back of rats, respectively. At 1, 2 and 3 weeks after implantation, histological changes and implant degradation were observed by hematoxylin-eosin staining. RESULTS AND CONCLUSION： The small intestinal submucosa sponge, which was prepared by the small intestinal submucosa with the mass fraction of 1% and 100 mmol/L cross-linking agent, had elastic and close space structure, uniform pore size and regular structure, so it was selected as the implant into the muscle. At 1 week after implantation, in the test group, the mesh sponge had the complete structure with few neutrophils, lymphocytes and giant cell reaction, and soft tissue hyperplasia and migration surrounding the implant appeared; in the control group, there were numerous inflammatory cells, and wound adhesion and little migration of surrounding tissues could be found. At 3 weeks, inflammatory cells mostly disappeared, and fibroblast-like cells and vascular components appeared, with thinner and regular collagen fiber bundles, and connective tissue-like structures could be found. In contrast, the control group still had numerous inflammatory cells

关 键 词：生物相容性材料 交联试剂 组织工程 生物材料 材料相容性 小肠黏膜下基质海绵 形态学观察 孔径 交联 

分 类 号：R318[医药卫生—生物医学工程]