聚甲基丙烯酸甲酯水蛭素人工晶状体的生物相容性  

Biocompatibility of hirudin peptide-modified acrylic intraocular lens

作　　者：李素华[1] 张旖旎[2] 李剑波[1] 

机构地区：[1]南阳市中心医院,河南省南阳市473000 [2]南昌大学医学部基础医学院,江西省南昌市330000

出　　处：《中国组织工程研究》2016年第21期3150-3155,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:传统晶状体虽然能够改善患者视力,但是材料生物相同性较差,炎症异物反应发生率较高。目的:探讨聚甲基丙烯酸甲酯水蛭素人工晶状体的生物相容性,研究水蛭素多肽改性丙烯酸系人工晶状体在眼科疾病中的应用效果。方法:(1)采用浸泡法在活化后的丙烯酸表面构建水蛭素多肽的生物涂层,通过细胞学观察聚甲基丙烯酸甲酯水蛭素晶状体的生物相容性。(2)将6只新西兰大白兔随机等分为常规组和水蛭素多肽组。常规组新西兰大白兔植入常规晶体,水蛭素多肽组植入聚甲基丙烯酸甲酯水蛭素人工晶状体。结果与结论:(1)聚甲基丙烯酸甲酯水蛭素人工晶体在植入3 d后丙烯酸系人工晶体上细胞大量增殖,并且细胞形态铺展良好,而细胞膜上细胞较少且存在较多的呈球形未铺展状态;(2)水蛭素多肽组新西兰大白兔边缘攀附的细胞向人工晶状面迁移一些,但是并没有阻碍中心视野,而常规组依然覆盖者纤维化的细胞膜;与常规组相比,水蛭素多肽组植入后SchirmerⅠ试验值升高,而泪膜破裂时间减少;(3)结果提示聚甲基丙烯酸甲酯水蛭素人工晶状体具有优良的物化性能和生物相容性,将其运用于白内障中效果理想,能够改善视力,能够为白内障的治疗提供新的思路,具有较高的临床应用价值。BACKGROUND： Because of poor biocompatibility, conventional intraocular lenses（IOLs） used to improve visual acuity can result in a higher incidence of inflammatory foreign body reactions. OBJECTIVE： To study the biocompatibility and therapeutic effects of hirudin peptide-modified acrylic IOL on eye diseases. METHODS： By immersion coating method, we prepared a polymethylmethacrylate IOL by the modification with hirudin polypeptides and observed its biocompatibility through the cytological observation. In this study, six New Zealand white rabbits were equivalently randomized into conventional and hirudin peptide grouppolypeptide hirudin group. Rabbits in the conventional group were given conventional lens implantation; while those in the hirudin peptide group underwent hirudin peptide-modified polymethylmethacrylate IOL. RESULTS AND CONCLUSION： Three days after implantation of hirudin peptide-modified polymethylmethacrylate IOL, cells proliferated and spread well on the acrylic IOL, but on the cell membrane, there were a few cells most of which were gathered spherically with no spreading. In the hirudin peptide group, some cells attached to the border zone migrated to the IOL surface, but did not impede the central visual field; in the conventional group, the IOL surface was still covered by fibrotic cell membrane. Compared with the conventional group, the score in the Schirmer I test was increased but the breakup time of tear film decreased in the hirudin peptide group. To conclude, the polymethylmethacrylate IOL modified by hirudin peptides has good physicochemical properties and biocompatibility, increases visual acuity, and yields satisfactory outcomes in the cataract treatment, which provides a new insight into the treatment of cataract.

关 键 词：白内障 炎症 有晶状体眼人工晶状体植入术 组织工程 生物材料 材料相容性 水蛭素 丙烯酸 人工晶状体 生物相容性 物化性能 

分 类 号：R318[医药卫生—生物医学工程]