大肠埃希菌和肺炎克雷伯菌空间诱变后的毒力变化分析  

Changes of virulence of Escherichia coli and Klebsiella pneumoniae after space mutation

作　　者：王俊锋[1] 李天志[2] 张学林[1] 李佳[1] 方向群[1] 袁明[3] 刘长庭[1] 

机构地区：[1]解放军总医院南楼呼吸科,北京100853 [2]解放军总医院南楼医务部,北京100853 [3]中国航天员科研训练中心,北京100094

出　　处：《解放军医学院学报》2016年第6期644-646,共3页Academic Journal of Chinese PLA Medical School

基　　金：国家"973"重点基础研究发展规划项目课题(2014CB744404)~~

摘　　要：目的了解空间诱变对大肠埃希菌和肺炎克雷伯菌毒力的影响。方法选取大肠埃希菌地面对照菌株、空间诱变菌株和肺炎克雷伯菌地面对照菌株、空间诱变菌株,在LB培养基培养后配制成109 CFU/ml的菌液并稀释成不同浓度,每只动物腹腔注射0.5 ml,测定其对30日龄健康ICR小鼠的半数致死量(LD50)。结果大肠埃希菌空间诱变和地面对照菌株的LD50分别为10^(7.25) CFU/ml、10^(7.5) CFU/ml,肺炎克雷伯菌分别为10^(7.25) CFU/ml、10^(7.75) CFU/ml,大肠埃希菌和肺炎克雷伯菌的空间诱变菌株LD50均低于其地面对照菌株。结论空间诱变可使大肠埃希菌和肺炎克雷伯菌的毒力增强。Objective To investigate the changes of virulence of Escherichia coli and Klebsiella pneumoniae after space mutation. Methods Four bacterial strains including the ground control and the space mutation strain of Escherichia coli and Klebsiella pneumoniae were selected, and the median lethal dose（LD50） of them were determined after the mice（with the age of 30 d） were intraperitoneally injected with 0.5 ml of bacterial suspension at different dilution concentrations. Results The LD50 of the space mutation and ground control strain of Escherichia coli were 10^（7.25） CFU/ml and 10^（7.5） CFU/ml respectively, and those of Klebsiella pneumonia were 10^（7.25） CFU/ml and 10^（7.75） CFU/ml. The median lethal dose of the space mutation strain was lower than that of the ground control strain in both Escherichia coli and Klebsiella pneumonia. Conclusion Space mutation can enhance the virulence of Escherichia coli and Klebsiella pneumoniae.

关 键 词：半数致死量 大肠埃希菌 肺炎克雷伯菌 空间诱变 

分 类 号：R378.2[医药卫生—病原生物学]