中国广西龙虎山野生猕猴粪便的病毒宏基因组学分析  

Metagenomic Analysis of Wild Rhesus Monkey Virome in Longhu Mountain in Guangxi Area,China

作　　者：刘文彬[1] 张翠媛[1,2] 虞结梅[2] 李利利[2] 敖元云[2] 段招军[2] 

机构地区：[1]湖南师范大学生命科学学院蛋白质化学与鱼类发育生物学重点实验室,湖南长沙410081 [2]中国疾病预防控制中心病毒病预防控制所病毒性腹泻室,北京100052

出　　处：《病毒学报》2016年第3期273-282,共10页Chinese Journal of Virology

基　　金：国家自然科学基金(81290345);湖南省科技计划资助项目(2014FJ3084);湖南省教育厅资助科研项目(13C523)

摘　　要：非人灵长类携带的病毒种类繁多,其中部分对人具有致病性。为深入了解我国野生猕猴携带病毒的状况,本研究应用MiSeq高通量测序及生物信息学分析技术,对从广西采集的280份猕猴粪便标本进行了病毒宏基因组学的分析。高通量测序共获得了233 726 79条读长(Reads),其中4641条序列与病毒相关,进一步注释到27个病毒科(包含细小病毒科中的细小病毒亚科和浓核病毒亚科),包括其中5种脊椎动物病毒(占78.2%)、6种昆虫病毒(占5.5%)、11种植物病毒(占10.4%)、其他的病毒(占9.8%);遗传进化分析结果显示,被注释为萨佩罗病毒、肠道病毒、细小病毒、腺相关病毒等序列与已知病毒相似,部分序列呈现明显的差异;应用PCR扩增进一步证实了病毒序列的真实存在。本研究初步确定了广西地区猕猴粪便中病毒的病毒谱,为深入分析和研究其中有潜在公共卫生意义的病毒奠定了基础。Several viruses have been found in non-human primates,among which some are pathogenic to humans.To further characterize the spectrum of viruses present in wild rhesus monkeys,MiSeq high-throughput sequencing were used to analyze 280 fecal samples collected from Guangxi,China.A total of 23,372,679 reads were obtained,of which 4,641 were annotated to 27 viral families or subfamilies,including five vertebrate viruses families（78.2%）,six insect virus families（5.5%）,eleven plant virus families（10.4%）,and other viruses（9.8%）.Further analysis revealed that these reads best fit with the sapelovirus,enterovirus,parvovirus,adeno-associated virus read sequences shared a high similarity with the known viruses.However,some reads presented obvious differences from these viruses.Moreover,PCR amplification was conducted to confirm these potentially novel viruses.This study had explored the viral spectrum of rhesus monkey feces in the Guangxi area,which laid the foundation for the potential public health significance of these viruses.

关 键 词：病毒宏基因组学 猕猴 病毒组 高通量测序 

分 类 号：Q78[生物学—分子生物学] S852.65[农业科学—基础兽医学]