2014～2015年河南地区PRRSV的分子检测及NSP2、ORF5基因变异分析  

Molecular Identification and Variation Analysis of the NSP2 and ORF5 Genes of PRRSV in Henan Province from 2014 to 2015

作　　者：郭天准 常洪涛[1] 崔丹丹[1] 王新港[1] 周峰[1] 赵军[1] 杨霞[1] 李永涛[1] 陈陆[1] 王川庆[1] 

机构地区：[1]河南农业大学牧医工程学院,河南郑州450002

出　　处：《病毒学报》2016年第3期298-307,共10页Chinese Journal of Virology

基　　金：河南省高校科技创新团队支持计划(14IRTSTHN015)

摘　　要：为了解河南地区猪繁殖与呼吸综合征病毒（PRRSV）流行毒株的分子特征及变异情况,本研究对2014～2015年河南各地118个养殖场的250份PRRS疑似病料进行RT-PCR检测,并对PRRSV阳性样品的NSP2和ORF5基因进行测序及变异分析。结果显示,PRRSV阳性样品共58份,阳性率为23.2%;扩增得到29个NSP2基因序列和31个ORF5基因序列;遗传进化分析表明,河南地区PRRSV流行毒株主要为美洲型毒株,其中14株与高致病性PRRSV（HP-PRRSV）高度同源,其余15株与美洲毒株NADC30高度同源,且该类毒株的nsp2蛋白存在131个氨基酸的不连续缺失,与国内及韩国报道的新变异毒株具有相似的缺失特征。与2012～2013年河南地区PRRSV分子流行病学调查结果相比,2014～2015年河南地区PRRSV流行株主要以HP-PRRSV和NADC30-Like毒株为主,尤其是后者在流行毒株中所占比例急剧上升,并且PRRSV流行毒株的NSP2和ORF5基因均发生很大变异,提示有必要对PRRSV的流行和变异进行持续监测,并对其致病性等进行深入研究,从而为新型疫苗及抗病毒药物的研发提供科学的参考依据。This study aimed at investigating the molecular epidemiology and genetic variation of PRRSV based on the detection of 250 clinical samples collected from 118 farms from 2014 to 2015in different regions of Henan Province by RT-PCR.The NSP2 and ORF5genes of the PRRSV-positive samples were sequenced and analyzed.The results showed that 58 samples were positive for PRRSV,with a positive rate of 23.2%.A total of 29NSP2 and 31ORF5genes were obtained.The phylogenetic analysis revealed that most of the prevalent strains belonged to the North American genotype.Among the 58 positive samples,14 strains were highly homologous with HP-PRRSV,another 15 samples were highly homologous with the North American prevalent strain,and NADC30 contained a discontinuous deletion of 131 amino acids in Nsp2,which had been recently reported in China and Korea.This study showed that the HP-PRRSV and NADC30-Like strains are presently the dominant strains in Henan,particularly in comparison with the results from 2012 to 2013.Moreover,the NADC30-Like strains accounted for a higher percentage.In addition,both the NSP2 and ORF5genes had significant variations,suggesting that more attention should be continuously paid to monitor the pathogenic epidemiology and genetic variation of PRRSV.Furthermore,additional research should be conducted regarding the mechanism of pathogenicity and immunological suppression of PRRSV to provide a reference for the research and development of vaccines and antiviral drugs.

关 键 词：猪繁殖与呼吸综合征病毒 NSP2基因 ORF5基因 NADC30株 变异分析 

分 类 号：S852.65[农业科学—基础兽医学]