白细胞介素-21在不同牙周状态龈沟液中的表达及相关性分析  

The expression and correlation of IL-21 in GCF with different types of periodontitis

作　　者：刘迪昕[1] 高科北 靳赢[1] 方絮[1] 王玉[1] 林晓萍[1] 

机构地区：[1]中国医科大学附属盛京医院口腔科,辽宁沈阳110004

出　　处：《口腔医学》2016年第5期438-441,共4页Stomatology

基　　金：高等学校博士学科点专项科研基金(20112104110013);辽宁省科学技术计划项目(2012225015);沈阳市科学技术项目计划(F13-221-9-28)

摘　　要：目的检测白细胞介素(Interleukin,IL)-21在不同牙周状态下龈沟液中的表达,探讨IL-21在牙周免疫中的可能作用。方法本研究共纳入47例患者,分为健康对照组(15例),轻度牙周炎组(10例),中重度牙周炎组(22例);记录患者一般信息、用Florida牙周探针检查牙周袋探诊深度(PD)和临床附着丧失(CAL)情况;收集牙周治疗前的龈沟液样本,采用酶联免疫吸附法(ELISA)测定不同牙周条件下龈沟液中IL-21的水平;并进行Pearson秩相关检验统计IL-21的表达与相关临床指标的关系。结果 3组龈沟液中IL-21平均浓度分别为(107.20±2.54)、(218.90±5.11)、(367.80±7.27)pg/m L,轻度牙周炎组及中重度牙周炎组龈沟液中IL-21水平显著高于对照组(P<0.05),其中以中、重度牙周炎组增高最为显著;中、重度牙周炎组龈沟液中IL-21水平分别与PD、CAL呈正相关关系(P<0.001)。结论 IL-21在牙周疾病中有较高的表达,尤其是以中重度牙周炎患者最为显著;由此推测IL-21在牙周疾病的发生发展中占有重要地位,与牙周组织的破坏有密切关系。Objective To investigate the concentration of IL-21 in gingival crevicular fluid（ GCF） and the correlation between IL-21 and periodontal clinical parameters to discuss the possible role of IL-21 in different types of periodontitis. Methods 15 healthy people were included as control group,10 patients with slight periodontitis were selected as slight periodontitis group,and 22 patients with moderate or severe chronic periodontitis were selected as moderate or severe periodontitis group. The basic information was recorded,and probing depth（ PD） and clinical attachment level（ CAL） were detected by Florida periodontal probes. GCF was collected from patients in each group. The IL-21 protein in GCF was determined by Enzyme Linked Immunosorbent Assay（ ELISA）. Pearson rank correlation and SPSS19. 0 software was used for statistical analysis. Results The mean concentration of IL-21 protein in GCF of each group was（ 107. 20±2. 54） pg / m L,（ 218. 90±5. 11） pg / m L,and（ 367. 80±7. 27） pg / m L; The concentration of IL-21 in both periodontitis groups were significantly higher than that in the control group（ P〈0. 05）,and the highest concentration of IL-21 was demonstrated in the moderate or severe periodontitis group. There was significant positive correlation between IL-21 and PD or CAL（ P 0. 001）. Conclusion The IL-21 protein in GCF of periodontitis is high,especially in moderate or severe chronic periodontitis. IL-21 may play a vital role in periodontal disease,which is related with the destruction of periodontal tissues.

关 键 词：白细胞介素-21 龈沟液 慢性牙周炎 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]