HPLC法分离测定苦豆子愈伤组织喹诺里西啶生物碱含量及体系优化  

Separation and Determination of Quinolizidine Aalkaloids in Callus of Sophora alopecuroides L.by HPLC and Its System Optimization

作　　者：高媛[1] 李鹏[2] 张庆宸 徐全智[1] 孙牧笛 顾沛雯[1] 

机构地区：[1]宁夏大学农学院,宁夏银川750021 [2]宁夏大学化工学院,宁夏银川750021 [3]山东大学药学院,山东济南250100

出　　处：《北方园艺》2016年第10期122-126,共5页Northern Horticulture

基　　金：国家自然科学基金资助项目(31260452)

摘　　要：以苦豆子愈伤组织为试材,采用高效液相色谱法,分离并测定苦豆子愈伤组织中氧化槐果碱、氧化苦参碱、槐定碱、槐果碱和苦参碱,建立了这5种单碱分离、测定的色谱方法。结果表明:色谱条件为UltimateAQ-C18(4.6μm×250mm×5mm);流动相为0.01mol·L^(-1)磷酸缓冲液(K2HPO4=5.59g·L^(-1),KH2PO4=0.41g·L^(-1),pH 7.5)-甲醇(50∶50,V/V);检测波长为216nm;柱温为35℃;流速为1.0mL·min-1,检测苦豆子愈伤组织中含有5种生物单碱,其中氧化槐果碱和槐定碱在苦豆子愈伤组织继代培养第4代时含量相对较高,分别为1.205 6mg·g-1和0.116 5mg·g-1,氧化苦参碱、槐果碱、苦参碱的含量略有波动,大致呈下降趋势。Taking the callus of Sophora alopecuroides L.as the experimental materials,using the method of HPLC for the separation and determination of oxysophocarpine,oxymatrine,sophoridine,sophocarpine and matrine in callus of Sophora alopecuroides L.,a chromatographic method for the separation and determination of five kinds of alkaloids fromSophora alopecuroides L.was established.The results showed that,chromatographic conditions,UltimateAQ-C18（4.6μm×250mm×5mm）;mobile phase,0.01mol·L^（-1） phosphate buffer（K2HPO4=5.59g·L^（-1）,KH2PO4=0.41g·L^（-1）,pH7.5）-methanol（50∶50,V/V）;UV detection at 216nm;column temperature,35 ℃;flow rate,1.0mL·min-1.There were five kinds of alkaloids in the callus of Sophora alopecuroides L..The ingredients and contents of oxysophocarpine and oxymatrine were higher in subculture callus which were cultivated into stage 4.They were 1.205 6mg·g-1 and0.116 5mg·g-1,sophoridine,sophocarpine and sophocarpidine were fluctuated slightly and downtrended more or less as the time went by.

关 键 词：苦豆子愈伤组织 HPLC 氧化槐果碱 氧化苦参碱 槐定碱 槐果碱 苦参碱 

分 类 号：Q946[生物学—植物学]