巨魾细胞色素氧化酶基因多态性及系统进化研究  

Study on the polymorphism and phylogeny of cytochrome oxidase gene of Bagarius yarrelli

作　　者：杜民[1,2] 牛宝珍[1,2] 王婷婷[1,2] 艾加林 刘艳红[1,2] 

机构地区：[1]红河学院云南省高校农作物优质高效栽培与安全控制重点实验室,云南蒙自661199 [2]红河学院生命科学与技术学院,云南蒙自661199

出　　处：《上海海洋大学学报》2016年第3期337-343,共7页Journal of Shanghai Ocean University

基　　金：国家自然科学基金(31360638);云南省教育厅科学研究基金重大专项(ZD2013009);云南省中青年学术带头人后备人才项目(2015HB059);红河学院中青年学术带头人后备人才项目(2014HB0203);红河学院博士专项(14bs11)

摘　　要：采用PCR技术克隆得到12尾巨魾(Bagarius yarrelli)的细胞色素氧化酶Ⅰ(COⅠ)、细胞色素氧化酶Ⅱ(COⅡ)和细胞色素氧化酶Ⅲ(COⅢ)的基因序列。结果显示:巨魾COⅠ基因序列全长1 551 bp,12个个体共出现9个单倍型,13个突变位点;COⅡ基因序列全长691 bp,12个个体共出现5个单倍型,5个突变位点;COⅢ基因序列全长784 bp,12个个体出现7个单倍型,8个突变位点。通过最小进化法Minimum Evolution(ME)构建系统发育树分析显示,巨魾与14种鮡科鱼在序列上有一定的同源性,并且COⅠ、COⅡ和COⅢ都与中华纹胸鮡(Glyptothorax sinense)、福建纹胸鮡(Glyptothorax fukiensis)、三线纹胸鮡(Glyptothorax trilineatus)和长丝黑鮡(Gagata dolichonema)在同一个分支,表明巨魾与纹胸鮡属和黑鮡属有较近的亲缘关系。The gene sequences of cytochrome oxidase subunit Ⅰ, cytochrome oxidase subunit Ⅱ and cytochrome oxidase subunit Ⅲ of 12 individuals of Bagarius yarrellis were obtained by PCR technique and clone methods; MEGA5. 0 program was used to analyze their phylogeny and polymorphism. It can be seen from the results that the total length of COⅠ gene sequence was 1 551 bp,as well as 12 individuals showed 9haplotypes and 13 mutation sites; the total length of CO Ⅱ gene sequence was 691 bp,as well as 12 individuals denoted 5 haplotypes and 5 mutation sites; the total length of CO Ⅲ gene sequence was 784 bp,as well as 12 individuals showed 7 haplotypes and 8 mutation sites. Phylogenetic analysis reveals that B.yarrelli has a certain sequence homology with 14 species of Sisoridae. The structured Minimum Evolution（ ME） phylogenetic tree from sequences of COⅠ,COⅡ and CO Ⅲ showed that B. yarrelli exists in the same branch with Glyptothorax sinense,Glyptothorax fukiensis,Glyptothorax trilineatus and Gagata dolichonema,which indicates that B. yarrelli has a close genetic relationship with species of Glyptothorax and Gagata.

关 键 词：巨魾 COⅠ COⅡ COⅢ 序列分析 系统发育 

分 类 号：S917[农业科学—水产科学]