褐菖鲉发声肌中小清蛋白的特性及肌肉纤维的超微结构特性  

Characteristics of the parvalbumin and the ultrastructure in the sonic muscle of the marbled rockfish,Sebasticus marmoratus

作　　者：谢伟[1,2] 张旭光[1,2,3,4] 郭弘艺[1,2,3,4] 宋佳坤[1,2,3,4] 

机构地区：[1]上海海洋大学水产与生命学院,上海201306 [2]上海海洋大学海洋生物系统与神经科学研究所,上海201306 [3]上海海洋大学省部共建水产种质资源发掘与利用教育部重点实验室,上海201306 [4]上海海洋大学国际海洋研究中心,上海201306

出　　处：《上海海洋大学学报》2016年第3期367-373,共7页Journal of Shanghai Ocean University

基　　金：国家自然科学基金(41406150);国家重点基础研究发展计划(2011CB111608);上海海洋大学国际海洋研究中心(A-209-5-0802)

摘　　要：通过对褐菖鲉发声系统的研究,尤其是发声肌形态、小清蛋白含量和肌纤维超微结构等特征,发现褐菖鲉发声肌和白色肌肉中小清蛋白分子量均为10-14 ku。在白色肌肉肌纤维显微结构中,三联体（一个T小管＋两个肌质网终池）仅处于Z膜,而在发声肌纤维中,三联体不仅在Z膜处,也在A带与I带交联处。与白色肌相比,发声肌超微结构中的肌质网更宽,肌膜更发达。在发声肌细胞中,线粒体多且聚集;而白肌细胞中,线粒体相对少且分散。结果显示：在褐菖鲉发声肌的快速收缩中,小清蛋白可能没有起重要作用,而是发达的肌膜、三联体和肌质网结构确保了该特殊肌肉快速收缩和放松的发声功能与行为,同时,大量聚集的线粒体保证其发声肌的持续工作能力。This anatomical,biochemical and the ultrastructural study on the sound production system in marbled rockfish Sebasticus marmoratu is focused on characteristics of the parvalbumin and the ultrastructure of the sonic muscle. The results reveal that the molecular weight of parvalbumin is about 10 ~ 14 ku in both sonic muscle and the white muscle of the fish. The TEM micrograph reveals the ultrastructural characteristics of the muscle fibers,among that the T system/SR of the sonic muscle are typically at both the Z-lines and A/I junctions,but are at the level of the Z-line only in the white muscle fibers. Sarcoplasmic reticulum and cell membranes in the sonic muscle fibers are wider and more developed than those in the white muscle.Moreover,there are more mitochondria which are crowded together in the sonic muscle cell,by contrasting,that are relatively fewer and are scattered in the white muscle cell. Our results suggest that： in marbled rockfish,the parvalbumin may not be playing an important role in the fast contraction of the sonic muscle,but the wide sarcoplasmic reticulum and enhanced cell membrane,the T system/sarcoplasmic reticulum,these ultrastructural advances may ensure the rapid contraction function of the sonic muscles for sound production.The feature of large amount of crowded mitochondria in the sonic muscle cells may enable the fish to produce the sound.

关 键 词：褐菖鲉 发声肌 小清蛋白 三联体 

分 类 号：S917.4[农业科学—水产科学] Q959.4[生物学—动物学]