青蒿琥酯分别与诺氟沙星、甲硝唑伍用的体内、外抗恶性疟作用  

IN VITRO AND IN VIVO EFFECT OF ARTESUNATE COMBINED WITH NORFLOXACIN OR METRONIDAZOLE AGAINST P. FALCIPARUM

作　　者：李春富[1] 杨恒林[1] 李丽[1] 李兴亮[1] 杨品芳[1] 高白荷[1] 

机构地区：[1]云南省寄生虫病防治所,云南思茅665000

出　　处：《中国寄生虫病防治杂志》2002年第4期208-210,共3页Chinese Journal of Parasitic Disease Control

基　　金：联合国计划开发署 /世界银行 /世界卫生组织热带病研究与培训特别规划处 (ID- 971 1 4 4 );中国云南省卫生厅 (No.980 0 7)资助项目~~

摘　　要：目的　了解青蒿琥酯分别与诺氟沙星、甲硝唑伍用的体内、外抗疟作用。　方法　采用青蒿琥酯与诺氟沙星 (A组 )或甲硝唑 (B组 )联用 3d疗法治疗无并发症的恶性疟。体外测定采用 Rieckmann体外微量法测定恶性疟原虫对 3种药物单一用药及青蒿琥酯分别与诺氟沙星或甲硝唑联用的敏感性。　结果　体内观察法共收治 70例病人 ,其中 A组 5 5例 ,B组 15例。平均退热时间分别为 (2 6 .5± 16 .5 ) h(8h～ 93h)、(19.2± 11.0 ) h(4h～ 4 1h) ;平均原虫无性体转阴时间分别为 (37.4± 15 .3) h(13h～ 93h)和 (42 .8± 14 .7) h(2 5 h～ 72 h) ;2 8d复燃率分别为 4 7.4 %和 75 .0 %。体外微量法测得青蒿琥酯与诺氟沙星伍用的 ID50 分别为单用组的 5 .9%和 0 .3% ;青蒿琥酯与甲硝唑伍用的 ID50 分别为单用组的38.8%和 5 .6 %。　结论　青蒿琥酯分别与诺氟沙星、甲硝唑伍用在体外对抗青蒿琥酯恶性疟原虫有明显增效作用 。Objective To understand in vitro and in vivo effect of artesunate combined with norfloxacin or metronidazole against Plasmodium falciparum . Methods By artesunate combined with norfloxacin or metronidazole to treat uncomplicated falciparum malaria patients by 3 day treatment method. By Rieckmanns in vitro assay to compare the results of the sensitivity of single drug use and combination drug use. Results 70 cases were treated by in vivo observation. Group A is 55 cases; Group B is 15 cases. The mean fever clearance time is (26.5±16.5) h (8 h-93 h), (19.2±11.0) h (4 h-41 h), the mean asexial parasite disappearance time is (37.4±15.3) h (13 h-93 h) and (42.8±14.7) h (25 h-72 h); In 28 d, the relapse rate is 47.4% and 75.0%, respectively. By micro in vitro assay, ID 50 of artesunate combined with metronidazole is 38.8%(33.0/85.1) and 5.6%(198.6/3587.6) of single drug use respectively. ID 50 of artesunate combined with norfloxacin is 5.9%(5.0/85.1) and 0.3%(34.0/9818.2) of the single drug use. Conclusion In vitro assay, artesunate combined with norfloxacin or metronidazole has an obvious increasing effect to artesunate resistant P. falciparum , but in clinic treatment, it can not improve the curative rate.

关 键 词：青蒿琥酯 诺氟沙星 甲硝唑 联合用药 恶性疟疾 实验研究 

分 类 号：R978.61[医药卫生—药品] R531.3[医药卫生—药学]