痹痛合剂对OA大鼠关节软骨形态学变化及软骨细胞Bcl-2基因表达的影响  

The Influence of BiTong Mixture on the Expression of Cartilage Cells Bcl-2 and Articular Cartilage Morphological Changes of OA Rats

作　　者：李素明[1] 杭柏亚[1] 

机构地区：[1]南京市中医院,江苏南京210001

出　　处：《西部中医药》2016年第5期18-22,共5页Western Journal of Traditional Chinese Medicine

基　　金：南京市医学科技发展项目(编号YKK12151)

摘　　要：目的:观察痹痛合剂对骨性关节炎(OA)关节软骨细胞形态学变化的影响并探讨其可能的作用机制。方法:将SD雄性大鼠64只随机分为假手术组(A组)、模型组(B组)、硫酸氨基葡萄糖组(C组)和痹痛合剂组(D组),A组行假手术,B、C、D组采用Hulth法复制OA模型,A组常规饲养,其余各组术后分别加服生理盐水,硫酸氨基葡萄糖溶液和痹痛合剂。术后第8周处死,分别大体观察、光镜400×和电镜下观察关节软骨细胞形态学变化,原位杂交法检测关节软骨细胞中Bc1-2 mRNA表达。结果:B组关节软骨呈典型OA形态学改变,关节软骨细胞中Bc1-2 mRNA表达减弱;C、D组关节软骨轻度退变,形态学方面与A组接近,关节软骨细胞中Bc1-2 mRNA表达显著增强,与B组比较差异有统计学意义(P<0.01)。结论:痹痛合剂与硫酸氨基葡萄糖能延缓OA大鼠关节软骨的退变,其可能的作用机制是通过提高关节软骨细胞中Bc1-2基因的表达。Objective： To observe the influence of BiTong mixture on articular cartilage morphology of osteoarthritis （OA） rats, and explore its possible mechanism. Methods： Sixty-four SD male rats were randomized into sham operation group（A）, the model group （B）, glucosamine sulfate group （C） and BiTong mixture group （D）, the rats in group A were performed with sham operation, the rats in the group B, C and D were replicated by Hulth method, the rats in the group A accepted conventional feeding, the rats in other groups took physiological saline, glucosamine sulfate solution and BiTong mixture after the operation respectively. The rats were sacrificed at the eighth week after the operation, cellular morphology of articular cartilage was inspected by gross observation, under light microscope and electron microscope, and the expressions of Bcl-2 mRNA in articular cartilage cells were detected by in situ hybridization （ISH）. Results： Articular cartilage showed typical OA morphological changes in the group B, the expressions of Bcl-2 mRNA in articular cartilage cells reduced obviously; articular cartilage degenerated slightly in the groups C and D, morphological changes were similar to the rats in the group A, the expressions of Bcl-2 mR- NA in articular cartilage cells enhanced significantly, it had significant difference compared with the group B （P〈0.01）. Conclusion： BiTong mixture and glucosamine sulfate solution could delay articular cartilage degeneration of OA rats, and the mechanism might be inhibiting articular cartilage cellular apoptosis by raising the expressions of Bcl-2 mRNA in articular cartilage cells.

关 键 词：骨性关节炎 软骨细胞 细胞形态学 B细胞淋巴瘤/白血病-2 痹痛合剂 

分 类 号：R285.5[医药卫生—中药学]