两株不同地区干巴菌菌丝体培养基的优化  

Optimized Mycelium Culture Medium of Two Strains Thelephora ganbajun from Different Regions in Yunnan

作　　者：王康康[1] 沙涛[1] 杨智[1] 普春龙 孙赵龙 

机构地区：[1]云南大学省生物资源保护与利用重点实验室,云南昆明650091

出　　处：《中国食用菌》2016年第3期33-36,共4页Edible Fungi of China

基　　金：国家自然科学基金项目(No.31260006);云南省高校科技创新团队

摘　　要：对分离自云南省玉溪市大营街镇平坝村及昆明市小哨乡的干巴菌菌丝体的最适营养条件及最适p H条件进行探究。通过单因子试验和正交试验优化培养基。结果表明,小哨菌种最适培养基为葡萄糖20 g、蛋白胨2 g、酵母粉2 g,磷酸二氢钾0.5 g、硫酸镁0.5 g,初始p H5.5,H_2O 1 L。玉溪菌种最适培养基为葡萄糖20 g、硫酸铵4 g、酵母粉2 g,磷酸二氢钾0.5 g、硫酸镁0.5 g,初始p H5.5,H_2O 1 L。The optimal culture medium and pH of two strains Thelephora ganbajun Zang from town Xiaoshao and Daying in Yunnan province were studied. The single factor and the orthogonal array method used for optimal culture medium. The results of these experiments showed that the components of the medium for Xiaoshao strains comprised with glucose at 20 g L-1, pep- tone 2 g L-1, yeast extract 2 g L-1, KH2PO4 0.5 g L-1, MgSO4 0.5 g L-1 and initial pH 5.5, for Yuxi strains the medium com- prised with glucose at 20 g L-1, yeast extract 2 g L-1, （NH4）2SO4 2 g L-1, KH2P04 0.5 g L-1, MgSO4 0.5 g L-l and initial pH 5.5.

关 键 词：干巴菌 菌丝 培养基 优化 

分 类 号：S646[农业科学—蔬菜学]