苦参碱对人膀胱癌BIU-87细胞增殖的抑制作用及其机制研究  

Study on the Inhibitory Effects of Matrine on the Proliferation of Human Bladder Cancer BIU-87 Cells and Its Mechanism

作　　者：姚莉[1] 武兴斌[1] 秦龙[1] 

机构地区：[1]兰州大学第二医院药学部,兰州730030

出　　处：《中国药房》2016年第16期2177-2180,共4页China Pharmacy

基　　金：中央高校基本科研业务费专项资金项目(No.lzujby-2013-216)

摘　　要：目的：研究苦参碱对人膀胱癌BIU-87细胞增殖的抑制作用及其机制。方法：以0（阴性对照）、0.5、1.0、2.0、4.0 mg/ml苦参碱分别作用于人膀胱癌BIU-87细胞24、48、72 h后,MTT法检测细胞活性并计算细胞增殖抑制率;以0（阴性对照）、0.5、1.0、2.0、4.0 mg/ml苦参碱作用于细胞48 h后,流式细胞术检测细胞周期和凋亡率,Western blot法检测细胞中生存素、天冬氨酸半胱氨酸蛋白酶（Caspase）-3及Caspase-7蛋白的表达。结果：与阴性对照比较,1.0-4.0 mg/ml苦参碱作用24、48、72 h后均对BIU-87细胞增殖有显著的抑制作用（P〈0.05或P〈0.01）,增殖抑制率呈浓度和时间依赖性升高;作用48 h后,G0/G1期细胞比例升高、S期及G2/M期细胞比例降低、凋亡率升高（P〈0.05或P〈0.01）。0.5-4.0 mg/ml苦参碱作用48 h后,细胞中生存素蛋白表达减弱、Caspase-3及Caspase-7蛋白表达增强（P〈0.05或P〈0.01）。结论：苦参碱可抑制人膀胱癌BIU-87细胞的增殖、阻滞细胞周期、诱导细胞凋亡,其机制可能与调控细胞中生存素及Caspase-3、Caspase-7的表达有关。OBJECTIVE：To investigate inhibitory effects of matrine on the proliferation of human bladder cancer BIU-87 cells and its mechanism.METHODS：The cell viability was detected by MTT assay and inhibitory rate of cell proliferation was calculated after human bladder cancer BIU-87 cells were treated with 0（negative control）,0.5,1.0,2.0 and 4.0 mg/ml matrine for 24,48 and 72 h,respectively.After treated with 0（negative control）,0.5,1.0,2.0 and 4.0 mg/ml matrine for 48 h,the cell cycle and apoptotic rate were detected by flow cytometry;the expression of Survivin,Caspase-3 and Caspase-7 protein were detected by Western blot assay.RESULTS：Compared with negative control,the proliferation of BIU-87 cells were significantly inhibited after incubated with 1.0-4.0 mg/ml matrine for 24,48 and 72 h（P〈0.05 or P〈0.01）,and inhibitory rate of cell proliferation increased in concentration and time-dependant manner;after treated for 48 h,the percentage of G0/G1 phase cells and apoptotic rate increased,while the percentage of cells at S phase and G2/M phase were decreased（P〈0.05 or P〈0.01）;the expression of Caspase-3 and Caspase-7 protein increased,while the expression of survivin protein decreased after incubated with 0.5-4.0 mg/ml matrine for 48 h.CONCLUSIONS：Matrine can inhibit the proliferation of human bladder cancer BIU-87 cells,block the cell cycle and induce apoptosis;its mechanism may be related to the expression regulation of Survivin,Caspase-3 and Caspase-7.

关 键 词：苦参碱 人膀胱癌BIU-87细胞 增殖 凋亡 

分 类 号：R285[医药卫生—中药学]