壮骨伸筋胶囊对维甲酸致小鼠骨质疏松的改善作用  

Improvement Effects of Zhuanggushenjin Capsules on Osteoporosis Induced by Retinoic Acid in Mice

作　　者：郭庆 孙晓丽 李丽娟 郝江叶 段重高 周亚伟 

机构地区：[1]北大世佳研究中心,北京102206

出　　处：《中国药房》2016年第16期2187-2189,共3页China Pharmacy

基　　金：吉林省科技发展计划项目(No.20130302025YY)

摘　　要：目的:考察壮骨伸筋胶囊对维甲酸致小鼠骨质疏松的改善作用。方法:将84只小鼠随机分为正常对照组、模型对照组、阳性对照组[骨疏康颗粒,5 g(生药)/kg]和壮骨伸筋胶囊高、中、低剂量组[7.80、3.90、1.95 g(生药)/kg]。除正常对照组外,其余各组小鼠均ig维甲酸(90 mg/kg)复制骨质疏松模型;造模7 d后,给药组小鼠ig相应药物,正常对照组和模型对照组小鼠ig生理盐水,每天1次,连续14 d。检测血清中碱性磷酸酶(ALP)活性及血清和股骨中钙、磷含量,检测小鼠体质量和股骨的干质量、骨长度和骨横径,并计算脏器(脾脏、睾丸、卵巢、子宫)系数。结果:与正常对照组比较,模型对照组小鼠血清中ALP活性升高,股骨中钙、磷含量以及小鼠体质量、股骨长度和股骨横径明显减少,脾脏系数升高,睾丸系数和子宫系数降低(P<0.05或P<0.01)。与模型对照组比较,壮骨伸筋胶囊各剂量组小鼠股骨中钙、磷含量增加,脾脏系数降低;高、中剂量组小鼠血清中ALP活性降低,小鼠体质量、股骨长度增加,睾丸系数和子宫系数升高;高剂量组小鼠股骨横径增加(P<0.05或P<0.01)。结论:壮骨伸筋胶囊对维甲酸致小鼠骨质疏松有一定的改善作用。OBJECTIVE：To investigate the improvement effects of Zhuanggushenjin capsules on osteoporosis induced by retinoic acid in mice.METHODS：84 mice were randomly divided into normal control group,model control group,positive control group [Gushukang granules,5 g（crude drug）/kg] and Zhuanggushenjin capsules high-dose,medium-dose and low-dose groups[7.80,3.90,1.95 g（crude drug）/kg].Except for normal control group,those groups were given retinoic acid（90 mg/kg）to induce osteoporosis model.7 days after modeling,all drug groups were given relevant medicine intragastrically,normal control group and model control group given normal saline intragastrically,once a day,for consecutive 14 days.ALP activity and the contents of calcium and phosphorus in serum and thigh-bone were determined.The body weight,the dry weight,length and diameter of thigh-bone were determined,and organ coefficients（spleen,testicle,ovarium and uterus）were calculated.RESULTS：Compared with normal control group,the activity of ALP in serum of mice increased in model control group,while the contents of calcium and phosphorus,body weight,length and diameter of thigh-bone decreased significantly;spleen coefficients increased,while testicle and uterus coefficients decreased（P〈0.05 or P〈0.01）.Compared with model control group,the contents of calcium and phosphorus increased in Zhuanggushenjin capsules groups,while spleen coefficient decreased;the activity of ALP in serum of mice decreased in high-dose and medium-dose groups,while body weight,the length of thigh-bone,testicle and uterus coefficients increased;diameter of thigh-bone increased in high-dose group（P〈0.05 or P〈0.01）.CONCLUSIONS：Zhuanggushenjin capsules can improve retinoic acid-induced osteoporosis in mice to certain extent.

关 键 词：壮骨伸筋胶囊 维甲酸 骨质疏松 改善 小鼠 

分 类 号：R285[医药卫生—中药学]