广藿香挥发油对感染后肠易激综合征模型大鼠结肠黏膜上皮细胞紧密连接蛋白ZO-1、Occludin表达的影响  

Effects of Volatile Oil of Pogostemon cablin on the Expression of Tight Junction Protein ZO-1 and Occludin in Colonic Mucosal Epithelial Cells of Rats with Post-infectious Irritable Bowel Syndrome

作　　者：刘瑶[1] 邓文辉 刘伟 

机构地区：[1]广东食品药品职业学院中医保健学院,广州510520 [2]广东省执业药师注册中心,广州510080 [3]东方药林药业有限公司,广州510515

出　　处：《中国药房》2016年第16期2190-2193,共4页China Pharmacy

基　　金：广东省中医药局科研项目(No.20131279)

摘　　要：目的:研究广藿香挥发油对感染后肠易激综合征(PI-IBS)模型大鼠结肠黏膜上皮细胞紧密连接蛋白ZO-1、Occludin表达的影响。方法:将大鼠随机分为正常对照组(蒸馏水)、模型组(蒸馏水)、马来酸曲美布汀片组(TMT,0.1 g/kg)和广藿香挥发油低、中、高剂量组[2、3、4 g(生药)/kg],每组8只。除正常对照组外,其余各组大鼠均采用结肠灌注乙酸法复制PI-IBS模型。造模成功后,每天ig给药1次,连续给药5 d。采用免疫组化法检测结肠黏膜中ZO-1、Occludin蛋白表达[以积分光密度(IOD)值反映]并分析其分布情况。结果:正常对照组大鼠ZO-1、Occludin蛋白均匀分布在肠上皮细胞的顶端,呈蜂窝状或呈点状聚集;模型组大鼠ZO-1、Occludin蛋白零散分布在肠上皮细胞的顶端,其染色分布不均或褪色;广藿香挥发油组大鼠ZO-1、Occludin蛋白分布类似正常对照组,但其染色浅于正常对照组。模型组大鼠ZO-1、Occludin蛋白的IOD值低于正常对照组(P<0.01);广藿香挥发油高剂量组大鼠ZO-1、Occludin蛋白的IOD值,以及广藿香挥发油中剂量组、TMT组大鼠ZO-1蛋白的IOD值高于模型组(P<0.05或P<0.01)。结论:广藿香挥发油可上调PI-IBS大鼠肠黏膜上皮细胞紧密连接蛋白ZO-1和Occludin的表达。OBJECTIVE：To study the effects of volatile oil of Pogostemon cablin on the expression of tight junction proteins ZO-1 and Occludin in colonic mucosal epithelial cells of rats with post-infectious irritable bowel syndrome（PI-IBS）.METHODS：Rats were randomly divided into normal control group（distilled water）,model group（distilled water）,Trimebutine maleate tablet group（TMT,0.1 g/kg）and volatile oil of P.cablin low-dose,medium-dose and high-dose groups [2,3,4 g（crude drug）/kg] with8 rats in each group.Except for normal control group,those groups were given colon perfusion of acetic acid to induce PI-IBS model.After modeling,they were given relevant medicine intragastrically once a day for consecutive 5 days.The expression of ZO-1and Occludin [by IOD] and their distribution were detected by immunohistochemistry.RESULTS：In normal control group,ZO-1and Occludin evenly distributed on the top of enterocyte,manifesting as alveolate and punctiform;in model group,ZO-1 and Occludin scattered on the top of enterocyte,showing uneven dyeing or fade;the distribution of ZO-1 and Occludin in volatile oil of P.cablin groups was similar to normal control group,and its dyeing was lighter than that of normal control group.IOD value of ZO-1and Occludin in model group were lower than in normal control group（P〈0.01）;those of volatile oil of P.cablin high-dose group and the IOD value of ZO-1 of P.cablin medium-dose group and TMT group were higher than those of model group（P〈0.05 or P〈0.01）.CONCLUSIONS：The volatile oil of P.cablin can up-regulate the expression of tight junction proteins ZO-1 and Occludin in colonic mucosal epithelial cells of rats with PI-IBS.

关 键 词：广藿香挥发油 感染后肠易激综合征 紧密连接蛋白 大鼠 

分 类 号：R285.5[医药卫生—中药学]