IKK抑制剂对大鼠颈动脉球囊损伤术后再狭窄的影响  

Influence of IKK Inhibitor on Carotid Artery Restenosis in Rat by Balloon Catheter Injury

作　　者：吴桂平[1] 曹伟[1] 张彦红[1] 周瀛[1] 邓会明[1] 邢俊[1] 

机构地区：[1]沈阳医学院附属第二医院心内科,辽宁沈阳110002

出　　处：《沈阳医学院学报》2016年第3期138-141,共4页Journal of Shenyang Medical College

基　　金：沈阳科技计划资金项目(No.130393)

摘　　要：目的:研究核转录因子κB抑制蛋白激酶(inhibitor of kappa B kinase,IKK)抑制剂对大鼠颈动脉球囊损伤后再狭窄的影响。方法:将32只雄性Wistar大鼠随机分为3组:假手术组、盐水组和药物组,盐水组和药物组建立大鼠颈总动脉球囊损伤模型。盐水组与假手术组均给予生理盐水注射,药物组给予IKK抑制剂注射,造模成功后15 d,采用HE染色观察血管内皮形成及内膜的增生情况,采用蛋白质印迹法检测核转录因子κB(NF-κB)、IKK、NF-κB抑制蛋白(inhibitor of nuclear factor kappa B,IκB)的表达情况,采用放射免疫法检测白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)的含量,采用基质金属蛋白酶1(MMP-1)检测试剂盒检测MMP-1的含量。结果:假手术组、药物组及盐水组大鼠颈总动脉内膜均逐渐增厚。假手术组和药物组与盐水组内膜面积,NF-κB、IKK、IκB表达水平,及IL-6、TNF-α、MMP-1含量比较差异均有统计学意义(P<0.05或0.01),假手术组和盐水组上述指标比较差异无统计学意义(P>0.05)。结论:IKK抑制剂通过抑制IKK-IκB-NF-κB通道所诱导的炎症反应,可以抑制血管损伤后再狭窄的发生。Objective： To investigate the influence of inhibitor of kappa B kinase （IKK） inhibitor on carotid artery restenosis in rat by balloon catheter injury. Methods： A total of 32 male Wistar rats were randomly divided into control group, injured group and medicine group. The balloon catheter injury was performed on left common carotid artery of rat by imitating the process of angioplasty. Injured group and control group were injected saline, medicine group were injected IKK inhibitor. After 15 days, the process of neointimal and media hyperplasia was observed by HE staining and the expression level of NF-κB, IKK, inhibitor of nuclear factor kappa B （IκB） was detected by Western blot, the content of IL-6, TNF-α was detected by radioimmunoassay, and the content of MMP-1 was determined by MMP-1 kit. Results： Arterial neointima hyperplasia hapened in 3 groups. There was significantly difference between control group and medicine group and injured group in intimal area, the expression level of NF-κB, IKK, IκB, and the content of IL-6, TNF-α and MMP-1 （P〈0. 05 or 0. 01）. There was no significance in these indexes between control group and medicine group （P〉0. 05）. Conclusion： IKK inhibitor can reduce the carotid artery restenosis after balloon injury by inhibiting inflammatory reaction induced by IKK-IκB-NF-κB pathway.

关 键 词：IKK IKK抑制剂 再狭窄 核转录因子ΚB 

分 类 号：R543[医药卫生—心血管疾病]