透钙磷石涂层浸提液掺锶对MC3T3-E1细胞成骨相关因子表达的影响  

The effects of brushite coating extract with strontium on the osteoblast-related factor expression of MC3T3-E1 cells

作　　者：文臣娟[1] 梁永强 韩东颖[3] 

机构地区：[1]开滦总医院,唐山063000 [2]华北理工大学口腔医学院 [3]唐山市中心血站

出　　处：《实用口腔医学杂志》2016年第3期325-330,共6页Journal of Practical Stomatology

基　　金：河北省教育厅重点项目(编号:ZD2015016)

摘　　要：目的:探讨透钙磷石与锶对成骨细胞的协同作用。方法:电化学沉积制备含透钙磷石涂层钛片,浸提法制备其浸提液,向配制好的浸提液中分别加入0.1%、0.5%和1%的锶。将MC3T3-E1细胞置于掺锶的浸提液中进行培养,检测不同含锶量的透钙磷石涂层浸提液中MC3T3-E1细胞活性、ALP活性,RT-PCR检测b FGF和VEGF mRNA表达。结果:含透钙磷石涂层钛片浸提中锶的浓度为0.1%、0.5%和1%时,培养的MC3T3-E1细胞增殖率、ALP活性、b FGF和VEGF mRNA表达均高于无锶对照组(P<0.05)。结论:掺锶透钙磷石对MC3T3-E1细胞成骨功能有促进作用。Objective： To study the effects of brushite coating with strontium on the osteoblast-related factor expression of MC3T3-E1 cells. Methods： Brushite coating on tinanium surface was prepared by electrochemical deposition and the extract of the coating with stronium at 0. 1%,0. 5% and 1% were prepared respectively. MC3T3-E1 cells were cultured with the extracts,cell proliferation,ALP activity,b FGF and VEGF mRNA were examined by MTT assay,ALP staining and RT-PCR respectively. Results： Strontium containing extracts promoted the proliferation,increased the ALP activity and mRNA expression of b FGF and VEGF（ P 0. 05） of MC3T3 cells. Conclusion： Brushite extract containing strontium may promote osteogenic function of MC3T3-E1 cells.

关 键 词：涂层 锶 透钙磷石 成骨细胞 

分 类 号：R318.08[医药卫生—生物医学工程]