TLR4基因Asp299Gly位点和Thr399Ile位点多态性与肺结核及结核性胸膜炎遗传易感性的研究  

STUDY ON THE ASSOCIATION BETWEEN ASP299GLY AND THR399ILE SINGLE NUCLEOTIDE POLYMORPHISMS OF TLR4 GENE AND THE SUSCEPTIBILITY OF PULMONARY TUBERCULOSIS AND/OR TUBERCULOUS PLEURITIS

作　　者：杨海波[1] 甘丽英[1] 谢恺庆[2] 

机构地区：[1]广西医科大学基础医学院微生物学教研室,南宁530021 [2]广西医科大学第一附属医院肾内科

出　　处：《广西医科大学学报》2016年第2期217-220,共4页Journal of Guangxi Medical University

基　　金：国家自然科学基金资助项目(No.81360243);广西壮族自治区教育厅自筹经费资助项目(No.201204LX057)

摘　　要：目的:探讨Toll样受体4(TLR4)基因Asp299Gly位点、Thr399Ile位点多态性与肺结核及结核性胸膜炎的遗传易感性。方法:采用错配聚合酶链反应—限制性片段长度多态性分析(PCR-RFLP)及PCR-直接测序法在190例结核患者(其中结核性胸膜炎患者64例)和110例健康自愿者人群(对照组)中检测TLR4基因Asp299Gly位点和Thr399Ile位点的突变频率,并对实验结果进行统计分析。结果:在结核组、结核性胸膜炎组和对照组中均未发现TLR4基因Asp299Gly位点和Thr399Ile位点的突变。结论:TLR4基因Asp299Gly位点和Thr399Ile位点多态性与肺结核及结核性胸膜炎的遗传易感性无关。Objective：To investigate the association between the Asp299 Gly and Thr399 Ile polymorphisms of the Toll-like receptor 4（TLR4）gene and susceptibility to pulmonary tuberculosis,and/or tuberculous.Methods：TLR4gene Asp299 Gly allele and Thr399 Ile allele mutation were screened by polymerase chain reaction-restricted fragment length polymorphism（PCR-RFLP）analysis technique in 190 pulmonary tuberculosis patients,including 68 tuberculous pleuritis patients and 110 healthy volunteers.Results：No mutation of TLR4 gene Asp299Gly allele and Thr399 Ile allele were detected in all 300 DNA samples.Conclusion：The non-synonymous single nucleotide polymorphism Asp299 Gly and Thr399 Ile in TLR4 gene was rare in Chinese population in Guangxi province,and had no association with the susceptibility to tuberculosis and/or tuberculous pleuritis.

关 键 词：TOLL样受体4 肺结核 结核性胸膜炎 单核苷酸多态性 

分 类 号：R521.7[医药卫生—内科学]