Expression of centromere protein-C(CENP-C) in villus tissue of the first-trimester spontaneous abortion and its correlation with chromosome segregation  

作　　者：Zhi-ping ZHANG Wei-wei XING Bin-bin ZHANG Li-xia LIANG Lei ZHU Xue-qing WU 

机构地区：[1]Reproductive Medicine Center, Children's Hospital of Shanxi & Women Health Center of Shanxi [2]Laboratory of Genome Project, Beijing Institute of Basic Medical Science [3]Department of Obstetrics and Gynecology, General Hospital of Taiyuan Iron & Steel Co., Ltd [4]Genetics Laboratory, Children's Hospital of Shanxi & Women Health Center of Shanxi

出　　处：《Journal of Reproduction and Contraception》2016年第1期18-24,共7页生殖与避孕（英文版）

基　　金：supported by the Natural Science Foundation of Shanxi province[2012021035-1]

摘　　要：Objective To investigate the expression of centromere protein-C （CENP-C） in villus tissue of the first-trimester spontaneous abortion （SA） and the correlation study of CENP-C expression with chromosome segregation. Methods Fluorescence in situ hybridization （FISH） and G-banded karyotype analysis were used to detect the numerical chromosome abnormality in 94 villus tissues of women with SA. The participants were separated into case group （n=30） and control group （n--30） according to the results with FISH. The qRT-PCR and Western blotting analysis were used to assess the expression level of CENP-C. Results Forty-eight （51.06%） cases had observed the numerical chromosome abnormality, including 30positive cases and the positive rate was 31.91%. The main types of variation included trisomy 16, 21, 22, X monosomy and triploid. The expression levels of CENP-C mRNA and protein in case group were statistically higher than that in control group （P〈0.05）. Conclusion Expression of CENP-C in the villus tissues of women might be related to SA induced by chromosomal aneuploid.Objective To investigate the expression of centromere protein-C （CENP-C） in villus tissue of the first-trimester spontaneous abortion （SA） and the correlation study of CENP-C expression with chromosome segregation. Methods Fluorescence in situ hybridization （FISH） and G-banded karyotype analysis were used to detect the numerical chromosome abnormality in 94 villus tissues of women with SA. The participants were separated into case group （n=30） and control group （n--30） according to the results with FISH. The qRT-PCR and Western blotting analysis were used to assess the expression level of CENP-C. Results Forty-eight （51.06%） cases had observed the numerical chromosome abnormality, including 30positive cases and the positive rate was 31.91%. The main types of variation included trisomy 16, 21, 22, X monosomy and triploid. The expression levels of CENP-C mRNA and protein in case group were statistically higher than that in control group （P〈0.05）. Conclusion Expression of CENP-C in the villus tissues of women might be related to SA induced by chromosomal aneuploid.

关 键 词：spontaneous abortion （SA） ANEUPLOIDY centromere protein-C （CENP-C） gene expression 

分 类 号：R714.21[医药卫生—妇产科学]