埃兹蛋白(ezrin)基因沉默抑制人前列腺癌PC-3细胞增殖和侵袭  

Silencing of ezrin gene inhibits proliferation and invasion of human prostate cancer PC-3 cells

作　　者：阳宁[1] 王玲[2] 陈仙[1] 刘俊[1] 罗志刚[1] 

机构地区：[1]南华大学附属第二医院泌尿外科,湖南衡阳421001 [2]湖南环境生物职业技术学院,湖南衡阳421001

出　　处：《细胞与分子免疫学杂志》2016年第6期821-824,828,共5页Chinese Journal of Cellular and Molecular Immunology

基　　金：湖南省科技厅基金(2015JC3088);衡阳市科技局基金(2010kj46)

摘　　要：目的观察埃兹蛋白(ezrin)在前列腺癌组织中的表达,探讨ezrin基因沉默对人前列腺癌PC-3细胞增殖、侵袭的影响及相关机制。方法收集前列腺癌和良性前列腺增生患者的标本各20例,采用荧光实时定量PCR检测ezrin mRNA水平、Western blot法检测ezrin蛋白水平。培养PC-3细胞,分别用培养液、Scramble siRNA、ezrin siRNA处理48 h;MTT法检测细胞增殖情况,TranswellTM侵袭实验检测细胞侵袭能力,荧光实时定量PCR、Western blot法检测上皮钙黏素、神经钙黏素的mRNA和蛋白水平。结果与良性前列腺增生患者的组织相比,前列腺癌组织中ezrin mRNA、蛋白表达水平均明显增加。与Scramble siRNA处理的PC-3细胞相比,ezrin表达下调后,PC-3细胞增殖受到明显抑制,平均穿膜细胞数、神经钙黏素水平降低,上皮钙黏素水平增加。Scramble siRNA处理的PC-3细胞与单纯培养液处理的PC-3细胞上述指标无明显差异。结论 Ezrin在前列腺癌组织中高表达,ezrin基因沉默能抑制PC-3细胞的增殖和侵袭,同时上调上皮钙黏素表达及下调神经钙黏素表达。Objective To observe the expression of ezrin in prostate cancer tissues and explore the effect of ezrin gene silencing on the proliferation and invasion of human prostate cancer PC-3 cells and involved mechanisms. Methods The paraffin-embedded specimens of prostate cancer（ n = 20） and benign prostatic hyperplasia（ n = 20） were collected.Real-time quantitative PCR and Western blotting were used to detect the expression of ezrin mRNA and protein in prostate tissues,respectively. After culturing,human prostate cancer PC-3 cells were treated with culture solution,Scramble siRNA and ezrin siRNA for 48 hours. Cell proliferation was analyzed through MTT method. Cell invasion ability was measured using TranswellTMinvasion experiment. In addition,the levels of E-cadherin and N-cadherin were examined by real-time quantitative PCR and Western blotting. Results The expression levels of ezrin mRNA and protein in prostate cancer tissues were higher than those in the tissues from benign prostatic hyperplasia patients. In comparison with PC-3 cells treated with Scramble siRNA,fol owing the downregulation of ezrin expression,PC-3 cel proliferation was significantly inhibited and mean cel number of penetrated membrane and N-cadherin level were reduced,whereas E-cadherin level was raised. There was no statistical difference in the above indicators between Scramble siRNA-treated PC-3 cells and pure culture solution-treated PC-3 cells.Conclusion Ezrin is highly expressed in prostate cancer tissues. Gene silence of ezrin inhibits the proliferation and invasion of human prostate cancer PC-3 cells,meanwhile the level of E-cadherin is upregulated and N-cadherin is downregulated.

关 键 词：前列腺癌 EZRIN 上皮钙黏素 神经钙黏素 

分 类 号：R392-33[医药卫生—免疫学] Q279[医药卫生—基础医学]