不同针刺刺激量对寒凝证类痛经大鼠疼痛反应及中脑内κ、μ受体mRNA表达的影响  

Effect of Different Acupuncture Stimulus on Pain Reaction and κ,μ Opioid Receptor mRNA Expressions in Midbrain of Dysmenorrheal Rats with Cold Stagnation Syndrome

作　　者：周瑾[1] 崔晓[1] 齐丹丹[1] 张梅[1] 刘珍珍[1] 郭孟玮[1,2] 赵雅芳[1,2] 任晓暄[1,2] 张露芬[1,2] 李晓泓[1,3] 朱江[1,3] 

机构地区：[1]北京中医药大学,北京100029 [2]国家中医药管理局针灸生物学三级实验室,北京100029 [3]国家中医药管理局针灸特色疗法评价重点研究室,北京100029

出　　处：《针灸临床杂志》2016年第5期78-81,共4页Journal of Clinical Acupuncture and Moxibustion

基　　金：国家重点基础研究发展计划(973计划)资助项目;编号:2012CB518506

摘　　要：目的:观察不同刺激量针刺关元穴对寒凝证类痛经大鼠扭体反应及中脑内κ、μ受体表达的影响,旨在讨论不同针刺刺激量(粗针、深刺、行手法与细针、浅刺、不行手法)对寒凝证类痛经模型大鼠的镇痛效应及其部分影响机制。方法:雌性大鼠40只,随机分成盐水组、寒凝类痛经模型组、刺激量A组(粗针、深刺、行手法)、刺激量B组(细针、浅刺、不行手法),每组10只。除盐水组外,其它各组采用冷冻法结合苯甲酸雌二醇加缩宫素制备类痛经大鼠模型,刺激量A组和刺激量B组以不同刺激量针刺关元穴。观察大鼠扭体反应,并以荧光定量PCR(RT-PCR)法测定中脑内κ、μ受体mRNA的表达水平。结果:与盐水组相比,模型组、刺激量A组以及刺激量B组扭体的潜伏期缩短(P<0.05),疼痛扭体次数、扭体评分显著增高(P<0.01)。与模型组相比,刺激量A组、刺激量B组的扭体潜伏期均明显延长(P<0.01),扭体次数、扭体评分显著减少(P<0.01)。结论:不同刺激量针刺关元穴对寒凝证类痛经大鼠均有镇痛作用;粗针、深刺、行手法针刺关元穴能够更好的缓解寒凝证类痛经大鼠的扭体反应。Objective：To investigate the analgesic effect and partly mechanism of the dysmenorrheal rats with cold stagnation syndrome by different acupuncture stimulus on CV4,observing the writhing reaction and the κ,μopioid receptor expression in midbrain.Methods：40 female SD rats were divided into four groups according to the random number（ n = 10）,including saline group,cold stagnation of primary dysmenorrheal model control group,A stimulus group（ thick needle,deep insertion,manipulated） and B stimulus group（ fine needle,shallow insertion,dismanipulated）.Other groups except saline group prepared the cold stagnation of primary dysmenorrheal models by combining systemic freezing and injection estradiol benzoate and oxytocin,different acupuncture stimulus in A stimulus group and B stimulus group.Observe the writhing reaction and the κ,μ opioid receptor expression in midbrain by RT-PCR.Results：The writhing latency decreased significantly,but the writhing times and writhing scores per minute obviously increased in the model group,A stimulus group and B stimulus group compared with the saline group（ P 〈 0.05）.Compared with the model group,writhing latency increased significantly,but the writhing times and writhing scores per minute obviously decreased in the A stimulus group and B stimulus group（ P 〈 0.01）.Conclusion：Different acupuncture stimulus on CV4 has an analgesic effect on the dysmenorrheal rats with cold stagnation syndrome.

关 键 词：寒凝证 类痛经 大鼠 扭体反应 κ、μ受体 

分 类 号：R246.3[医药卫生—针灸推拿学]