水稻水培抗倒伏相关性状的QTL分析  

QTL Analysis of Lodging Resistance-related Traits Under Hydroponic Culture in Rice

作　　者：王小虎[1,2,3] 方云霞[4] 祝阳舟 潘斌清 余海平[2] 张栋[2] 曾大力[2] 胡江[2] 钟卫国[3] 俞良[3] 端木银熙[3] 梁国华 钱前[2] 

机构地区：[1]扬州大学/江苏省遗传生理重点实验室/教育部植物功能基因组学重点实验室,江苏扬州225009 [2]中国水稻研究所/水稻生物学国家重点实验室,浙江杭州310006 [3]常熟市农业科学研究所/江苏省杂交晚粳工程技术研究中心/国家杂交水稻工程技术研究中心常熟分中心,江苏常熟215500 [4]江西省农业科学院水稻研究所,江西南昌330200

出　　处：《核农学报》2016年第5期850-858,共9页Journal of Nuclear Agricultural Sciences

基　　金：国家自然科学基金(31201194);江苏省科技计划后补助项目(BE2015378);江苏省苏州市科技计划项目(SNG201509)

摘　　要：为研究水稻抗倒伏相关性状的遗传机制,以典型的籼粳交(窄叶青8号/京系17)的F1花培加倍单倍体分离群体为材料,通过水培法在齐穗期考查了与水稻抗倒伏相关的根基粗、总根数、最大根长、根干物重和根冠比等地下部根系性状及分蘖数、株高、株周长、单蘖直茎和地上部干物重等地上部性状。利用该群体的分子连锁图谱进行QTL区间作图分析,除分蘖数、地上部干物重外,其它8个性状共检测到13个相关的QTL。其中与根基粗、根冠比、株周长、单蘖直茎相关的QTL各1个,分别位于第8、第1、第7和第8染色体上。与总根数、最大根长、根干物重相关的QTL各2个,分别位于第1和第2、第1和第6、第1和第12染色体上。与株高相关的QTL共3个,分别位于第1、第4和第8染色体上。相关分析表明,各根系性状相互之间存在着极显著正相关;除根冠比外,其它根系性状与地上部分蘖数、株高、株周长、单蘖直径和地上部干物重之间也存在显著或极显著正相关。多元回归分析表明,除分蘖数外,地下部根系性状与其它地上部性状存在着显著线性效应。本研究为深入理解水稻抗倒伏因子的遗传基础及制定抗倒伏性育种策略等提供了理论依据。In order to study the genetic mechanism of rice lodging resistance-related traits,a set of doubled haploid（ DH）lines derived from a cross between indica cultivar Zhaiyeqing 8 and a japonica variety Jingxi 17 were used to investigate the traits related to lodging resistance including root thickness（ BRT）,total root number（ TRN）,maximum root length（ MRL）,root dry weight（ RDW）,root shoot ratio（ RSR）,tiller number（ TN）,plant height（ PH）,plant perimeter（ PP）,single tiller diameter（ STD） and shoot dry weight（ SDW）. Using a constructed molecular linkage map,the QTL analysis was carried out at full heading stage under hydroponic culture and a total of thirteen QTLs were detected.Among them,only one QTL each for BRT,RSR,PP and STD was detected on chromosomes 8,1,7 and 8 respectively,two QTLs each for TN（ on chromosomes 1 and 2）,MRL（ on chromosomes 1 and 6） and RDW（ on chromosomes 1 and12）,and three QTLs for PH on chromosomes 1,4 and 8 were detected. The highly significant positive correlations were observed between every two root traits by correlation analysis,and the aboveground traits of TN,PH,PP,STD and SDW also exhibited significant or highly significant positive correlation with root traits except for RSR. In addition,the multiple regression analysis also showed that there were significant linear effects between root traits and other aboveground traits of PH,PP,STD and SDW. These results will facilitate theoretical studying for further understanding the genetics basis of rice lodging resistance factor and formulating lodging resistance breeding strategy.

关 键 词：水稻 水培 抗倒伏性状 加倍单倍体 数量性状座位 

分 类 号：S511[农业科学—作物学]