GM-CSF浓度梯度对树突状细胞体外诱导培养的影响  

Effect of different concentrations of GM-CSF on biological behavior of dendritic cells cultured in vitro

作　　者：谢楠[1] 张理超[1] 付必莽[1] 唐波[1] 李春满[1] 赵越[1] 胡明道[1] 张捷 

机构地区：[1]昆明医科大学第二附属医院肝胆胰外科一病区,云南省昆明市650101

出　　处：《世界华人消化杂志》2016年第13期2038-2044,共7页World Chinese Journal of Digestology

基　　金：教育部博士点新教师基金资助项目;No.201053171200022010;年度云南省自然科学基金资助项目;No.2010ZC127~~

摘　　要：目的:观察不同浓度粒-巨噬细胞集落刺激因子(granulocyte-macrophage colonystimulating factor,GM-CSF)联合白介素4(interleukin,IL-4)对小鼠骨髓源性树突状细胞(dendritic cell,DC)形态、纯度、细胞数目的影响,探究树突状细胞诱导培养的最适宜条件.方法:分离、纯化小鼠骨髓单个核样细胞,分别加入不同浓度梯度的GM-CSF和定量的IL-4,对其进行形态学观察和细胞表型检测,以及对异基因淋巴细胞的激活作用测定.结果:在一定浓度范围内,随着GM-CSF浓度的增加,小鼠骨髓源性树突状细胞的数目、纯度及成熟度亦随之增加.而过高浓度的GM-CSF反而导致抑制作用.据此,可推测理想的GM-CSF浓度范围应为10-20 ng/mL.结论:适宜浓度的GM-CSF联合IL-4可提升小鼠骨髓单个核样细胞向树突状细胞分化的能力,两种细胞刺激因子的合理运用,可增加树突状细胞的数目、纯度及成熟度.AIM：To investigate the effect of granulocytemacrophage colony stimulating factor（GMCSF）with interleukin（IL）-4 on the morphology,purity,and number of mouse bone marrowderived dendritic-like cells,in order to explore the optimal conditions for culturing dendritic cells.METHODS：Mouse bone marrow mononuclear cells were dissociated and purified,and then different concentrations of GM-CSF with IL-4were added.The morphology and phenotype of dendritic-like cells were assessed,and their ability to activate allogeneic lymphocytes was measured.RESULTS：In a certain concentration range,with the increase of the concentration of GM-CSF,the number,purity and maturity of mouse bone marrow-derived dendritic cells increased,but high concentrations of GM-CSF had an inhibitory effect.The optimal GM-CSF concentration range was 10-20 ng/mL.CONCLUSION：Optimal concentrations of GM-CSF and IL-4 can be combined to enhance the ability to induce murine bone marrow mononuclear cells to differentiate into dendritic cells.Rational use of these two factors canincrease the number,purity and maturity of dendritic cells.

关 键 词：树突状细胞 体外培养 粒-巨噬细胞集落刺激因子 白介素-4 

分 类 号：R392[医药卫生—免疫学]