甘肃主栽小麦品种及骨干亲本花药培养特性评价及分析  

Evaluation and Analysis of Anther Culture Characteristics in Gansu Wheat Cultivars and Backbone Parents

作　　者：王炜[1] 陈琛[1] 叶春雷[1] 贺小宝[2] 杜旺喜 王云贵[2] 杨芳萍[3] 杨文雄[3] 杨随庄[4] 王方[1] 罗俊杰[1] 

机构地区：[1]甘肃省农业科学院生物技术研究所,甘肃兰州730070 [2]兰州职业技术学院生物工程系,甘肃兰州730070 [3]甘肃省农业科学院小麦研究所,甘肃兰州730070 [4]西南科技大学生命科学与工程学院,四川绵阳621010

出　　处：《核农学报》2016年第6期1059-1066,共8页Journal of Nuclear Agricultural Sciences

基　　金：甘肃省农科院农业科技创新基金(2013GAAS27);甘肃省农科院科技创新工程学科团队(2014GAAS06);甘肃省农业生物技术研究与应用开发项目(GNSW-2014-15)

摘　　要：为进一步提高小麦花培育种效率,拓宽花培杂交亲本可利用的基因型,以86份甘肃主栽小麦和骨干亲本为供试材料,利用花药培养技术,分析并评价其花药培养特性,探讨所筛选的具有高花药培养力基因型材料的应用潜力。结果表明,在供试小麦材料中,春小麦较冬小麦更易于诱导愈伤组织并分化出绿苗;愈伤组织诱导率与绿苗分化率及白苗分化率之间不具有相关性;绿苗生产率可作为评价小麦花药培养力的重要指标。此外,86份材料中共筛选出9份具有较高花药培养力的材料。研究结果可为利用甘肃小麦种质资源进行花培育种提供参考依据,同时所筛选出的高花药培养力材料在花培育种、DH群体构建、小孢子培养和无性系变异机理等基础研究方面具有重要的潜在应用价值。For further improving the efficiency of wheat anther-culture breeding and broadening the genotypes of crossing parents potentially applited in anther culture, the anther culture characteristics were evaluated and analyzed with 86 Gansu wheat cultivars and backbone parents by using anther culture technology; the application potential were discussed in the screened high-anther-culture-ability genotypes. The results indicated the spring wheat genotypes were easier to be inducted to form callus and then differentiate into green plantlets than that of winter wheat ones in this study; there was no correlation between the callus induction frequencies and green plantlet or albino plantlet differentiation frequencies; the green plantlet production frequency could be used as an important index in evaluating anther culture ability. The study will not only provide reference for wheat anther-culture breeding in using Gansu wheat germpalsm, but the selected high-anther-culture-ability genotypes are of important potential application value in wheat anther culture breeding, DH population establishment, microspore culture, and fundamental research.

关 键 词：小麦 花药培养特性 花药培养力 绿苗生产率 基因型 

分 类 号：S512.1[农业科学—作物学]