八棱海棠MdPPO2B基因的克隆及降解苯酚研究  

Cloning and Phenol Degradation of Polyphenol Oxidase Gene Md PPO2B From Malus Robusta Rehd.

作　　者：沈雪芳[1,2] 韩晴[2] 高建杰[3] 彭日荷[3] 王波[3] 高志红[1] 姚泉洪[1,3] 章镇[1] 

机构地区：[1]南京农业大学园艺学院,江苏南京210095 [2]上海市农业科学院作物育种栽培研究所,上海201403 [3]上海市农业科学院生物技术研究所,上海201106

出　　处：《核农学报》2016年第6期1091-1097,共7页Journal of Nuclear Agricultural Sciences

基　　金：上海市种业发展项目(沪农科种字(2013)第13号);上海市科学技术委员会科研计划项目(153919N1300);上海市市级农口系统青年人才成长计划[沪农青字(2015)第1-4]

摘　　要：为了探索果树对酚类化合物的修复潜力,从八棱海棠中克隆获得多酚氧化酶基因Md PPO2B,并构建该基因植物超量表达载体,通过转化模式植物拟南芥研究该基因对酚类化合物的修复功能。结果表明,在平板、盆栽、液体3种试验中,不加入苯酚,转基因植株在生长状态和外部形态上和野生型相似;当苯酚浓度分别为0.1 m M和1 m M时,通过植物的根长、叶片鲜重等比较发现,转基因株系比野生型植株对苯酚的耐受性更强,其生长形态较为正常,而野生型植株的生长则受到了明显抑制。由此可见,Md PPO2B转基因植物的PPO活性在植物降解酚类化合物过程中起重要作用;使用果树来源的多酚氧化酶基因用于有机污染物修复,对环境治理具有较好的应用前景。To explore the potential use of fruit tree in environmental renovation of phenolic compounds, we have cloned a polyphenol oxidase gene MdPPO2B from Malus Robusta and constructed a transgenic vector for over expressing this gene in plant. MdPPO2B was genetically transformed into the model plant Arabidopsis thaliana, and its function in degradation of phenolic compounds was analyzed. In solid MS, soil, liquid MS media without adding phenol, the morphology and growth status were indistinguishable between the transgenic and the wild type plants. However, when treated with the same media by adding 0.1 mM and 1 mM phenol respectively, MdPPO2B transgenic plants showed significantly enhanced tolerance to phenol compared to wild type. The root length and leave fresh weight of transgenic plants were longer and heavier than that of wild type plants. It indicates that the PPO activity, conferred by MdPPO2B, is highly required for degrading of phenol in transgenic Arabidopsis. This work suggests that many fruit-derived genes may be good candidate for phytoremediation of pollutants in the environment.

关 键 词：拟南芥 八棱海棠 苯酚 植物修复 

分 类 号：S661.4[农业科学—果树学]