头孢哌酮-舒巴坦联合中药对泛耐药鲍曼不动杆菌抗菌活性的研究  

Antibacterial efficiency of cefoperazone-sulbactam combined with traditional Chinese medicine against pan-drug resistant Acinetobacter baumannii

作　　者：谭俊青[1] 李蔼文[1] 王康椿 黄双旺[1] 周兵[1] 

机构地区：[1]广东省第二中医院检验科,广东广州510095

出　　处：《检验医学》2016年第5期350-354,共5页Laboratory Medicine

基　　金：广东省中医药局科研课题(20141020)

摘　　要：目的通过体外及体内抗菌实验,观察中药颗粒剂与头孢哌酮-舒巴坦的联合作用对泛耐药鲍曼不动杆菌(PDR-AB)的抗菌效果。方法采用不同浓度头孢哌酮-舒巴坦与8种中药颗粒剂(五倍子、黄连、黄芩、薄荷、连翘、乌梅、五味子及大黄)相结合,通过计算各自单用药最低抑菌浓度(MIC)及部分抑菌浓度指数(FIC)观察其对36株PDR-AB的体外抑菌效果,选取适当的方案分组后通过检测中性粒细胞、肿瘤坏死因子-α(TNF-α)及降钙素原(PCT)研究其对PDR-AB感染小鼠的抑菌作用。结果头孢哌酮-舒巴坦联合五倍子、黄连及黄芩具有协同作用,联合五味子、薄荷及连翘具有相加作用,联合乌梅和大黄显示为无关作用,且头孢哌酮-舒巴坦联合五倍子比单用药能更显著地降低感染小鼠的中性粒细胞数量、TNF-α及PCT水平。结论头孢哌酮-舒巴坦和五倍子联用对PDR-AB有明显的抑菌作用,可用于治疗由PDR-AB引起的重症感染。Objective To observe the antibacterial effi ciency of cefoperazone-sulbactam combined with traditional Chinese medicine against pan-drug resistant Acinetobacter baumannii（PDR-AB） in vitro and in vivo. MethodsDifferent concentrations of cefoperazone-sulbactam and traditional Chinese medicine（gallnut,coptis,scutellaria,mint,fructus forsythia,fructus mume,schisandra chinensis and rheum officinale） were used combinedly. Through calculating minimal inhibitory concentration（MIC） and fractional inhibitory concentration（FIC）,the antibacterial efficiency in vitro of 36 isolates of PDR-AB was observed,and the appropriate method was chosen to evaluate the treatment for bacterial-infected mice by determining neutrophil,tumor necrosis factor-alpha（TNF-α）and procalcitonin（PCT）. Results Cefoperazone-sulbactam combined with gallnut,coptis and scutellaria had synergized effect,while combined with schisandra chinensis,mint and fructus forsythia, it had additive effect. When combined with fructus mume and rheum offi cinale, it showed antagonised effect,and it combined with gallnut could signifi cantly reduce the levels of neutrophil,TNF-α and PCT. Conclusions Cefoperazone-sulbactam combined with gallnut has antibacterial effi ciency for PDR-AB.

关 键 词：泛耐药鲍曼不动杆菌 头孢哌酮-舒巴坦 中药 

分 类 号：R378.99[医药卫生—病原生物学]