黄精对AD模型大鼠空间学习记忆及α7 nAChR表达的影响  

Effects of polygonatum sibiricum on spatial learning and memory and the expression of α7 nAChR in AD model rats

作　　者：未小明[1] 王爱梅[1] 罗朝辉[2] 陈亚奇[1] 

机构地区：[1]南阳医学高等专科学校,南阳473061 [2]南阳医学高等专科学校第二附属医院,南阳473000

出　　处：《神经解剖学杂志》2016年第3期391-396,共6页Chinese Journal of Neuroanatomy

基　　金：河南省重点科技攻关项目(132102310263)

摘　　要：目的:探讨黄精对AD模型大鼠空间学习记忆能力及前额叶皮质和海马α7 n AChR表达的影响。方法:成年雄性SD大鼠随机分为四组:对照组、模型组、黄精低剂量组、黄精高剂量组。皮下注射D-半乳糖联合双侧海马注射Aβ25-35构建AD模型大鼠,黄精低、高剂量组同时每天分别给予低剂量(15 g/kg/d)或高剂量(30g/kg/d)的黄精水煎剂灌胃治疗,连续6周。Morris水迷宫测试大鼠的空间学习和记忆能力;免疫组织化学技术检测大鼠前额叶皮质和海马α7 n AChR的表达。结果:Morris水迷宫结果显示,模型组大鼠的逃避潜伏期(EL)与对照组相比明显延长(P<0.01),在目标象限游泳时间和穿越站台次数减少(P<0.01);与模型组比较,黄精低剂量组、高剂量组的EL缩短(P<0.01),在目标象限游泳时间和穿越站台次数增多(P<0.01);黄精高剂量组的EL较低剂量组缩短(P<0.05或P<0.01),在目标象限游泳时间和穿越站台次数差异无统计学意义(P>0.05)。免疫组化结果显示,模型组大鼠前额叶皮质和海马α7 n AChR表达水平较对照组明显降低(P<0.01);黄精低剂量组、高剂量组大鼠前额叶皮质和海马α7n AChR表达水平较模型组明显上调(P<0.05或P<0.01);黄精高剂量组大鼠α7 n AChR表达水平较低剂量组大鼠增多(P<0.05)。结论:黄精可以明显改善AD模型大鼠的空间学习记忆能力,其作用机制可能与调节α7 n AChR表达有关。Objective： To investigate the effects of polygonatum sibiricum on spatial learning and memory and the expression of α7 n AChR in the prefrontal cortex and hippocampus in Alzheimer＇s disease（ AD） model rats. Methods： Adult male SD rats were randomly divided into four groups： control group,model group,low-dose drug group,high-dose drug group. AD model rats were established by subcutaneous injection of D-galactose and injection of Aβ25-35 in both sides of hippocampus. Meantime,the rats were daily treated by intragastric administration of low doses（ 15 g / kg） and high doses（ 30 g / kg） of water extract of polygonatum sibiricum for 6 weeks. After the treatment,the spatial learning and memory was examined by using Morris Water Maze test. Then,α7n AChR protein expression in the prefrontal cortex and hippocampus was detected with immunocytochemistry. Results： Morris Water Maze test results revealed that the model rats exhibited significant increase in escape latency（ EL）（ P〈0. 01） but a decrease in the time of staying in quadrant of platform and the number of crossing over a platform as compared to control group（ P〈0. 01）. However,the EL was significantly decreased（ P〈0. 01） and the time of staying in quadrant of platform and the number of crossing over a platformwas remarkably longer（ P〈0. 05 or P〈0. 01） in the low-dose drug group and high-dose drug group than that in the model rats. Moreover,compared to the low-dose drug group,the EL was remarkably shorter（ P〈0. 05 or P〈0. 01） in the high-dose drug group but there were no significant differences on the time of staying in quadrant of platform and the number of crossing over a platform between the low-dose drug group and the high-dose drug group（ P〈0. 05）. The immunohistochemistry results showed that the expression of α7 n AChR was significantly decreased in the model rats compared with control group（ P〈0. 01）. Whereas the distributions of α7 n AChR was remarkably increased in the low-dose drug

关 键 词：黄精 阿尔兹海默病 空间学习记忆 Α7烟碱型乙酰胆碱受体 皮质和海马 大鼠 

分 类 号：R285.5[医药卫生—中药学]