胎儿生长受限VEGFA和VEGFR2在胎盘中的表达  

Expression of vascular endothelial growth factor a and its receptor in FGR-affected placental tissues

作　　者：孟茜[1] 陈海霞[1] 孙波[1] 张芳 骆秀翠[1] 

机构地区：[1]连云港市妇幼保健院产科扬州大学医学院附属医院,222006

出　　处：《中国妇产科临床杂志》2016年第3期241-243,共3页Chinese Journal of Clinical Obstetrics and Gynecology

基　　金：江苏省妇幼保健科研项目(F201237)

摘　　要：目的检测胎儿生长受限(FGR)患者胎盘血管内皮生长因子(VEGFA)及其受体(VEGFR2)的表达,终末绒毛微血管超微结构的改变。方法通过Realtime-PCR、Western-blot和免疫组化,检测FGR胎盘VEGFA和VEGFR2的表达;通过透视电镜分析FGR胎盘终末绒毛微血管的结构。结果 FGR组VEGFA和VEGFR2的mRNA水平(1.28±0.48)、(1.73±0.29)和蛋白水平(0.71±0.32)、(0.61±0.25)明显低于正常妊娠组(4.21±1.72)、(3.71±0.89)(P<0.01);1.90±0.76、1.75±0.48(P<0.01);免疫组化分析FGR组VEFGA和VEGFR2均为低表达;FGR胎盘终末绒毛微血管呈退行性变。结论 FGR患者胎盘VEFGA和VEGFR2水平显著下降,可能是导致FGR发病机制的一个重要因素。Objective The aim of this study was to examine the expression of vascular endothelial growth factor A（VEGFA）and receptor-2（VEGFR2）in fetal growth restriction（FGR）affected placental tissue and their functional effects on the villi microvascular.Methods Expression of VGFA and VEGFR2 were determined by realtime PCR,immunohistochemistry and western blot.The effects of VEGF ligands on microvascular of terminal villi in placentas was determined on the transmission electron microscopy（TEM）.Results Expression of VEGFA and VEGFR2 genes on mRNA and protein level were significantly decreased in the FGR group.Immunohistochemical analysis showed reduced expression of VEGFA and VEGFR2 protein.The degenerative alterations of the terminal villi vascular appeared in the FGR-affected placenta.Conclusions The placental mRNA expression and protein production of VEGFA and VEGFR2 were reduced in GFR.There is evidence of a biologically plausible mechanism by which VEGF family may contribute to the etiology of this important pregnancy disorder of FGR.

关 键 词：血管内皮生长因子 受体 胎儿生长受限 胎盘 

分 类 号：R714.5[医药卫生—妇产科学]