有氧呼吸条件下粪肠球菌LD33高密度增殖的研究  

Study of high density culture of Enterococcus faecalis LD33 undergoing aerobic respiration

作　　者：焦月华[1,2] 张兰威[1] 刘飞[3] 张爽[3] 

机构地区：[1]哈尔滨工业大学化工与化学学院,哈尔滨150090 [2]黑龙江中医药大学药物安全性评价中心,哈尔滨150040 [3]东北农业大学食品学院,哈尔滨150030

出　　处：《中国乳品工业》2016年第5期12-14,39,共4页China Dairy Industry

基　　金：国家自然科学基金(31401512);黑龙江省教育厅科学技术研究项目(12541768)

摘　　要：为获得高活菌数的粪肠球菌LD33的发酵液,首先通过优化粪肠球菌LD33的有氧呼吸代谢培养条件,随后利用单因素和正交实验对粪肠球菌LD33的高密度增殖培养基组分进行研究。结果表明,粪肠球菌LD33的最优增殖培养基为乳清60 g/L,葡萄糖30 g/L,蛋白胨20 g/L和酵母粉10 g/L(均为质量浓度);当接种量为1%,初始p H值为6.4且转速为220 r/min时,在37℃下培养24 h后,发酵液中粪肠球菌LD33的活菌数可以达到6.37×10~9m L^(-1)。In order to prepare highly concentrated Enterococcus faecalis LD33 cell culture, at first optimization of fermentation conditions was carried out, then single factor test and orthogonal test was applied to find the optimal high density proliferation medium of Enterococcus faecalis LD33. Results showed that the optimal medium for proliferation of Enterococcus faecalis LD33 cells was composed of 60 g/L whey, 30 g/L glucose, 20 g/L peptone and 10 g/L yeast powder. And when the inoculation amount was 1%, the initial p H was 6.4 and the rotational speed of shaking incubator was 220 r/min, the number of viable bacteria cell of Enterococcus faecalis LD33 in the fermentation broth could reach 6.37×10~9CFU/m L after cultivated at 37°C for 24 h.

关 键 词：粪肠球菌 高密度培养 有氧呼吸 

分 类 号：Q93-335[生物学—微生物学]