回顾性研究:猪超数排卵若干相关问题  

Retrospective study: several related problems s of porcine superovulation

作　　者：杨博超[1] 马喜山[1] 肖冲[1] 王谦[1] 刘牧[1,2] 

机构地区：[1]中国医学科学院医学实验动物研究所,北京100021 [2]中国疾病预防控制中心,北京100050

出　　处：《黑龙江畜牧兽医》2016年第6期82-87,共6页Heilongjiang Animal Science And veterinary Medicine

基　　金："十二五"国家科技支撑计划项目(2014BACK02B01)

摘　　要：本文采用回顾性研究方法,统计了截至2014年6月份在国内三大学术网站检索的中文文献及相关部分英文文献的资料、数据,对若干猪超数排卵（简称超排）问题进行探讨和归类总结。重点关注了供体猪的品种差异、超排适时年龄、超排时间与供体猪发情期、超排季节等因素对超数排卵的影响,还包括不同超排方案、不同超排试剂剂量组合的影响,并描述了超排后卵母细胞或胚胎采集的相关问题,对目前我国常用的猪超排程序进行了评价。结果表明：供体母猪品种不同,品种间超排效果有一定差异;不同年龄段猪超排,初情期前小猪对超排试剂相对敏感,但胚胎发育率及胚胎移植效果比青年母猪差。目前,我国猪超排大多采用孕马血清促性腺激素（PMSG）＋人绒毛膜促性腺激素（hCG）或前列腺素（PG）＋PMSG＋hCG方案。PMSG的首次注射时间为供体母猪前1个发情期后第15~16d,应用上述方案进行超排,PMSG一般用量为1000~1200IU,hCG用量为500~750IU;根据所需,按照胚胎的发育期适当调整卵母细胞或胚胎的采集方法和时间。This paper uses a method of retrospective study to count the materials and data of Chinese literature and the relevant part of English literature searched from three largest academic websites in China until June in 2014, and discusses, classifies and summarizes several related problems of porcine superovulation. The paper focuses on the effects of the varietal difference of donor pigs, timely age of superovulation, super- ovulation time, the oestrus of donor pigs, superovulation season, and other factors on the superovulation, as well as the effects of different schemes of porcine superovulation and different dosage combinations of superovulation reagents. It describes the related problems for collecting the oocytes or embryos after superovulation, and evaluates the porcine superovulation procedure commonly used in China at present. The results show that there axe some differences in the superovulation effects between the porcine varieties with different varieties of donor pigs ; the prepu- bertal piglets are more sensitive to the superovulation reagents on the superovulation at different ages, but the embryo developmental rates and the embryo transfer effects of piglets are lower than those of young sows. The scheme of pregnant mare serum gonadotropin （PMSG） plus human chorionie gonadotropin （hCG） or prostaglandin （PG） plus PMSG with hCG, is mostly used on porcine superovulation in China at present. The time of first injection of PMSG is at 15 - 16 d after the last estrus of donor sows, and the doses of PMSG and are generally in 1 000 to 1 200 IU and 500 to 750 IU using the above scheme of porcine superovulation, respectively. The collecting methods and time of the oocytes or embryos as needed should be adjusted according to the developmental periods of embryos.

关 键 词：回顾性研究 文献检索 猪 超数排卵 品种差异 发情期 

分 类 号：S814[农业科学—畜牧学] S828[农业科学—畜牧兽医]