四川省一个新引进外种猪群体氟烷基因及酸肉基因的检测和分析  

Detection and analysis of the HAL and RN genes from a new introduced foreign swine population in Sichuan province

作　　者：范源[1] 梁婵[1] 王睦群 巫小倩 张顺华[1] 朱砺[1] 

机构地区：[1]四川农业大学动物科技学院,成都611130

出　　处：《黑龙江畜牧兽医》2016年第6期91-94,共4页Heilongjiang Animal Science And veterinary Medicine

基　　金："十二五"国家科技支撑计划项目(2013BAD20B07);四川省科技支撑计划项目(2013NZ0041);科技部和四川省科技富民强县项目

摘　　要：为了加强对四川省某核心种猪场新引进种猪的选育,试验采用PCR-RFLPs方法对新引进的457头外种猪的氟烷(HAL)基因和酸肉(RN)基因的多态性进行研究。结果表明:新引进种猪中存在3头HALn基因携带者,HALn基因频率为0.33%。RN-rn+、RN-RN-基因型种猪数分别为1头、38头,在整个群体中RN-的基因频率为8.42%。在今后的育种工作中,应加强对氟烷基因和酸肉基因的检测和标记辅助选择(MAS),以选育出抗应激的优质猪群体。To strengthen the selective breeding of new introduced swines from a core breeding farms in Sichuan, the polymorphisms of halothane （HAL） gene and rendement napole （RN） gene from 457 foreign swines were studied using PCR - RFLPs method. The results showed that there were only 3 swines with HALn gene in the new foreign swine group, and the gene frequency of HAL＂ was 0.33%. The numbers of swines with RN-m＋ and RN- RN- genotypes were 1 and 38 respectively, and the gene frequency of RN- in the entire swine population was 8.42%. Therefore, the detection and marker- assisted selection （MAS） for the Hal gene and RN genes should be strengthened so as to selec- tively breed anti - stress pig populations with high quality in future breeding work.

关 键 词：种猪 PCR-RFLPS 标记辅助选择(MAS) 氟烷(HAL)基因 酸肉(RN)基因 

分 类 号：S828[农业科学—畜牧学] Q78[农业科学—畜牧兽医]