白头翁汤方及其单味药水提取液对猪、鸡大肠杆菌体外抑菌活性的测定  

Determination of in vitro bacteriostatic activity of the water extracts from Baitouweng decoction prescription and the single herbs against swine and chicken Escherichia coli

作　　者：徐倩倩[1,2] 郭时金[1,2] 王艳萍[1,2] 张志美[1,2] 杨丽梅[1,2] 张颖[1,2] 沈志强[1,2] 

机构地区：[1]山东省滨州畜牧兽医研究院山东省滨州畜禽蜂胶疫苗研究开发推广中心,山东滨州256600 [2]山东绿都安特动物药业有限公司,山东滨州256600

出　　处：《黑龙江畜牧兽医》2016年第6期185-187,191,共4页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然科学基金项目(31402243)

摘　　要：为了探讨白头翁汤方及其单味药水提取液对鸡、猪大肠杆菌的体外作用,试验采用微量二倍稀释法和平板法测定最小抑菌浓度(MIC)和最小杀菌浓度(MBC)。结果表明:黄连对大肠杆菌的抑杀作用最强,所有菌株对黄连都中度敏感(33.25 g/L≤MIC≤250.00 g/L),黄连对所有菌株也表现出明显杀菌效果(67.50 g/L≤MBC≤500.00 g/L);其次是白头翁,除了鸡大肠杆菌O78和猪大肠杆菌C83644表现不敏感外(MIC=500.00 g/L),其他菌株都中度敏感(67.50 g/L≤MIC≤125.00 g/L),白头翁对大肠杆菌也有杀菌作用(125.00 g/L≤MBC≤500.00 g/L);黄柏对大肠杆菌未表现出明显的抑菌、杀菌活性(250.00 g/L≤MIC≤500.00 g/L,MBC≥250.00 g/L);所有菌株对秦皮都不敏感(MIC≥250.00 g/L,MBC≥500.00 g/L);所有菌株对白头翁汤提取液都不敏感,白头翁汤中含有抗菌成分,但对细菌的抑杀作用并不是其发挥抗细菌性腹泻作用的唯一途径。To investigate the in vitro bacterlostatic activity of the water extracts from Baitouweng decoction prescription and the single herbs against swine and chicken Escherichia coli, micro - double dilution method and plate method were used to determine the minimum inhibitory concentration （MIC） and minimum bactericidal concentration （MBC）. The results showed that Coptis chinensis Franch had the strongest inhibiting and killing effects against Escherichia coli among the tested herbs, and all strains were moderately sensitive to Coptis chinensis Franeh. （33.25 g/L ≤ MIC ≤250. 00g/L）, and Coptis chinensis Franch. also exhibited significant bactericidal effects against all strains （67.50 g/L ≤MBC ≤ 500.00 g/L） ; followed by was PulsatiUa chinensis （Bunge） Regel, , all the other stains were moderately sensitive to Pulsatilla chinensis （Bunge） Regel （67.50 g/L≤MIC≤125. 00 g/L） except that Escherichia coli O78 from chickens and C83644 from pigs were insensitive （ MIC = 500.00 g/L）, while Pulsatilla chinensis （Bunge） Regel also had a bactericidal effect（ 125.00 g/L ≤ MBC ≤500.00 g/L） ; Phellodendron chinense Schneid. didnt show any significant baeteriostatic or bactericidal activity （ 250.00 g/L ≤ MIC ≤500.00 g/L, MBC ≥250.00 g/L ） ; while all stains were insensitive to Cortexfraxini and Baitouweng decoction （ MIC ≥250. 00 g/L, MBC ≥500.00 g/L）. The results indicate that Baitouweng decoction contains antibacterial ingredients, but the inhibiting and killing effects of Baitouweng decoction on bacteria are not the single way that it plays a role in anti - bacterial diarrhea.

关 键 词：白头翁汤 大肠杆菌 最小抑菌浓度 最小杀菌浓度 微量二倍稀释法 平板法 

分 类 号：R284.2[医药卫生—中药学] S852.612[医药卫生—中医学]