家畜免疫球蛋白表达谱研究进展  

Research Progress of Livestock Immunoglobulin Gene Expression Repertoire

作　　者：杜丽娟[1] 王淑辉[1] 朱艳娇[1] 康孟阳 李青旺[1] 孙秀柱[1] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100

出　　处：《家畜生态学报》2016年第5期78-84,共7页Journal of Domestic Animal Ecology

基　　金：中央高校基本科研业务费(Z109021424);西北农林科技大学引进人才科研启动基金项目(Z111021203)

摘　　要：目前,对于家畜的抗病育种工作正由传统育种转向分子育种领域,但对于家畜分子免疫领域,尤其是在免疫系统中起着重要决定性作用的免疫球蛋白基因的结构、类型和表达多样的分子机制相关研究报道较少,本文综述了常见家畜的免疫球蛋白表达谱研究进展。概括来说牛、羊和猪免疫球蛋白重链恒定区均含有编码五种免疫球蛋白的基因,但在猪和羊基因座上的排布情况还未确定。可变区的V、D和J片段数目相对于人和小鼠来说比较有限,其中羊的D片段未知。λ和κ两种轻链在三种动物中的使用状况有所不同:牛和羊偏好使用λ轻链,猪λ∶κ表达比率接近1∶1。Currently,traditional livestock resistance breeding methods are transforming to molecular ways.But the reports on molecular fields of livestock,especially on the structure,type of immunoglobulin genes which plays a decisive role in immune system,and on its expression diversity mechanism are very rare.This paper reviewed the immunoglobulin gene expression repertoires of common livestock.The immunoglobulin heavy chain constant region of ovine,bovine and swine generally contained five immunoglobulin genes,however,the organization of swine and ovine genes was unclear.Compared with human and mice,the number of V,D and J genes in variable regions was limited,with D fragment of ovine unknown.Lambda and kappa light chains were different in three livestock with different ratio：bovine and ovine preferred to express lambda chain,whereas,the ratio was close to 1∶1in swine.

关 键 词：家畜 免疫球蛋白 基因 多样性 表达谱 

分 类 号：S811.5[农业科学—畜牧学]