复发性实验性自身免疫性葡萄膜炎小鼠模型的诱导及其特点  

Induction and features of relapsing experimental autoimmune uveoretinitis in mice

作　　者：徐磊[1] 潘永全[1] 何明忠[1] 高杰[1] 陈飞兰[1] 

机构地区：[1]重庆医科大学实验动物中心重庆市啮齿类实验动物工程技术研究中心,重庆400016

出　　处：《第三军医大学学报》2016年第12期1432-1436,共5页Journal of Third Military Medical University

基　　金：国家自然科学基金青年科学基金(81200677);重庆市教委科学技术研究项目(KJ120303);重庆市科学技术委员会社会事业与民生保障科技创新专项(CSTC2015 shmszx120031)~~

摘　　要：目的诱导复发性实验性自身免疫性葡萄膜炎(experimental autoimmune uveoretinitis,EAU)小鼠模型,评价其发生过程及特点。方法完全弗氏佐剂(CFA)乳化的光感受器间维生素A类结合蛋白(IRBP)161-180肽段免疫B10.RⅢ小鼠作为诱导组(35只),用CFA-PBS免疫小鼠作为对照组(6只)。小鼠免疫后7、14、21、28、35 d裂隙灯显微镜和光学显微镜观察及评价眼部炎症发生、眼部表现和病理学变化;待炎症完全消失时,再次免疫小鼠,评价小鼠眼部炎症复发。结果诱导组首次免疫后7 d出现初发炎症,14 d炎症达高峰,随后炎症消退,至35 d完全消失。第35天进行再次免疫,36 d出现复发炎症,42 d达炎症高峰,随后炎症消退,但至观察期第96天部分鼠(1/5)仍维持炎症。眼部表现为睫状充血、前房渗出、瞳孔受损。病理学表现为视网膜和脉络膜炎性细胞浸润,血管炎、肉芽肿性炎,光感受器细胞层破坏,视网膜下渗出。对照组未见明显炎症。结论建立的EAU呈现慢性复发性病程,其眼部表现和病理学特征与人类葡萄膜炎相似,可作为葡萄膜炎研究的新型动物模型。Objective To induce and evaluate the onsets and features of relapsing experimental autoimmune uveoretinitis（ EAU） in B10. R Ⅲ mice. Methods B10. R Ⅲ mice of the experimental group were immunized with interphotoreceptor retinoid-binding protein（ IRBP） 161- 180 peptides in complete Freund＇s adjuvant（ CFA）,while the mice of the control group were immunized with CFA-PBS. The clinical findings and histopathologic changes of both groups were observed at weekly intervals for 5 weeks after primary immunization. At the resolution of inflammation,the mice underwent secondary immunization with IRBP-CFA or CFA-PBS to evaluate the inflammation relapse. Results The primary inflammation signs were observed on the 7th day,rose to the peak on the 14 th day,followed by gradual regression,and disappeared on the 35 th day after primary immunization. The mice underwent secondary immunization on the 35 th day after primary immunization. The relapsing inflammation signs were observed on the 36 th day,and then rose to the climax on the 42 nd day. The inflammation was alleviated on the 50 th day,but about 1 /5 of the mice still maintained inflammation signs on the 96 th day after primary immunization. The main clinical findings in the mice were ciliary injection,corneal opacity,fibrinous exudates in the anterior chamber,and lesions of the pupil. The prominent histopathologic lesions included inflammatory cellular infiltration in the retina and choroid,retinal vasculitis and granuloma,disorganization and loss of photoreceptors,and subretinal exudates. Conclusion The relapsing EAU mouse model is characterized with chronic course and clinical and histopathologic findings similar to human uveitis,so it can be used as a suitable model for research of human uveitis.

关 键 词：实验性自身免疫性葡萄膜炎 表型 病理学 复发 动物模型 

分 类 号：R332[医药卫生—人体生理学] R593.2[医药卫生—基础医学]