栗疫菌Cfcc82606菌株非蛋白毒素基本性质的研究  

Basic Properties of Nonprotein Toxin Produced by Endothia parasitica

作　　者：汤兴宇 宋晓斌[1] 张丹[1] 

机构地区：[1]西北农林科技大学林学院,陕西杨凌712100

出　　处：《中国南方果树》2016年第3期42-48,共7页South China Fruits

基　　金：“北方经济林主要病虫无公害防治技术”国家“十二五”科技支撑计划项目(2012BAD19B0804)资助

摘　　要：为了探索板栗疫病的致病机制,将具有致病力的栗疫菌菌株Cfcc82606(Endothia parasitica)的滤液,采用甲醇沉淀和离心得到蛋白和非蛋白类部分,经透析袋以及致病活性检测,该毒素为非蛋白类小分子毒素。对非蛋白类进行不同温度、不同pH值、紫外线照射、甲醇沉淀处理以及透析袋后,测定其致病活性的变化。结果表明:毒素有着较强的热稳定性,经121℃,0.103 MPa处理30min后仍有一定的致病性;毒素的酸碱性敏感,酸性条件下毒性增强,碱性条件下毒性减弱;紫外线的照射对毒素具有削弱作用。经7种不同极性物质萃取后,正丁醇相的致病力最强,该毒素为极性较大的物质。栗疫菌菌株Cfcc82606的非蛋白类毒素对同科属的植物都有着不同程度的致病作用,是一种非寄主类专化性毒素。In order to explore the pathogenic mechanism of chestnut blight, the crude protein toxin and non- protein toxin were prepared by methanol precipitation from the filtrate of Cryphonectria parasitica （Cfcc82606）. The toxin was a small molecule, non-protein substance determined by the dialysis bag and patho- genic activity detection. The changes of pathogenic activity of crude toxin after the treatments with different temperatures, pH values, ultraviolet irradiation and methanol precipitation were determined in order to under- stand the basic physicochemical properties of the toxin. The results showed that the toxin had a strong thermal stability and still remained a strong pathogenic activity after treatment at 121 ℃, 0. 103 Mpa for 30 mins. The non-protein toxin was sensitive to pH change. Its toxicity reduced under alkaline conditions and ultraviolet ra- diation. Seven polar substances used for extraction had different effects on the activity of non-protein toxin. The n-butyl alcohol extraction gave the highest activity of the toxin. Furthermore, the toxin had different de- grees of pathogenicity on the seedling growth of different plant species, indicating that the non-protein toxin was a non-host specific toxin.

关 键 词：栗疫菌 毒素 基本性质 致病活性 寄主专化性 

分 类 号：S436.64[农业科学—农业昆虫与害虫防治]