一种基于EDTA脱钙法的改良骨组织脱钙方法  

An Improved Method of Bone Decalcification Based on EDTA Decalcification Method

作　　者：赵雨坤 郑康[2,3] 郭晴晴[2,3] 樊丹平 何小鹃[2,4] 吕爱平[1,2,4] 

机构地区：[1]上海中医药大学,上海201203 [2]中国中医科学院中医临床基础医学研究所,北京100700 [3]西南交通大学生命科学与工程学院,成都610031 [4]香港浸会大学中医药学院,香港999077

出　　处：《中国实验方剂学杂志》2016年第11期17-19,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家"重大新药创制"科技重大专项(2013ZX09301307);国家自然科学基金项目(81470072);中国中医科学院自主选题项目(Z0252;Z0293;Z0412)

摘　　要：目的:探索操作方法简便、脱钙时间更短且对骨组织伤害较小的脱钙方法。方法:DBA/1小鼠40只,随机分组为胶原诱导关节炎模型组和正常组,并对模型组小鼠建立胶原诱导关节炎模型。分别取模型组和正常组踝关节,使用改良前后的脱钙方法进行脱钙,从关节病理及其评分、免疫组织化学方面进行比较脱钙效果。结果:经改良后,在脱钙温度4℃和脱钙液更换周期24 h条件下,脱钙速度较高(7 d),且此时对骨组织结构和骨组织内蛋白表达无明显影响。结论:优化的螯合剂脱钙法可以有效提高脱钙速度,可为快速制备病理诊断用骨切片提供了参考。Objective： To explore a decalcification method with easier operation and shorter time.Method： Forty DBA /1 mice were randomly divided into normal group and model group. Mice in model group were induced with type II collagen. Ankles were achieved from both model group and normal group,and each of groups were demineralized by improved method and conventional method, respectively. Effect of these two decalcification methods was compared from three aspects, including joint pathology, pathological scores and immunohistochemistry. Result： When the temperature was 4 ℃ and the replacement cycle of decalcification fluid was 24 h,the improved decalcification speed was 7 d,and this operation did not have an obvious effect on the structure and the protein expression of bone tissue. Conclusion： This optimized decalcification method can effectively improve the decalcification speed,which can get pathological diagnosis rapidly.

关 键 词：骨组织切片 乙二胺四乙酸二钠脱钙 胶原诱导关节炎 免疫组织化学 脱钙温度 

分 类 号：R945[医药卫生—微生物与生化药学] R283.6[医药卫生—药剂学]