诃子不同提取物的近红外光谱研究  

FTIR Spectroscopy of Differents Extracts of Chebulae Fructus

作　　者：李静怡[1] 李文兰[2] 马芳[3] 图雅[4] 

机构地区：[1]哈尔滨商业大学生命科学与环境科学研究中心,哈尔滨150076 [2]哈尔滨商业大学药学院,哈尔滨150076 [3]清华大学,北京100084 [4]中国中医科学院中医药发展研究中心,北京100700

出　　处：《中国实验方剂学杂志》2016年第11期72-76,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金面上项目(81274192)

摘　　要：目的:对诃子原药材、水提取物、无水乙醇提取物、石油醚提取物及主要有效成分鞣酸的红外光谱图进行了整体分析。方法:采用傅里叶变换红外光谱法(FTIR)并结合二阶导数谱和二维相关红外光谱技术(2D-IR)。结果:诃子药材及其提取物有着各自稳定的光谱特征,谱图中1 714 cm^(-1)的α,β-不饱和酯键的红外吸收峰,1 613,1 447 cm-1的苯环骨架振动是判断鞣酸在不同诃子样品中特征峰强弱的主要依据。诃子药材经过提取,以鞣酸为代表的主要成分得到有效富集,3种提取物中鞣酸的特征峰明显较强,其中水提取物中鞣酸的特征峰强度明显高于无水乙醇提取物和石油醚提取物。结论:红外光谱宏观指纹技术可提供大量的整体结构信息,能够为主要有效成分的提取分离及准确把握诃子药材的整体质量提供参考依据。Objective： To conduct a global analysis on the infrared spectrum of Chebulae Fructus raw material,water extract, absolute ethanol extract, petroleum ether extract and the main component tannin.Method： Fourier transform infrared spectroscopy（ FTIR）, second-derivative infrared spectroscopy and twodimensional correlation infrared spectrum（ 2D-IR） were adopted. Result： Chebulae Fructus raw material and extracts had respective stable spectral characteristics. The infrared spectrum of α,β-unsaturated ester bonds at 1714 cm^-1,and aromatic skeleton vibration at 1 613,1 447 cm^-1were the main basis to determine the characteristic peak intensity of tannin in different Chebulae Fructus samples. After extraction,the main component tannin was effectively enriched. The characteristic peak of tannin was stronger in the three extracts,and the intensity of characteristic peak of tannin in water extract was higher than that in absolute ethanol extract,petroleum ether extract. Conclusion： The infrared spectrum macroscopical fingerprint technology could provide large amount of information on the overall structure,and provide reference for the extraction and separation of the main effective ingredients and accurately controlling the overall quality of Chebulae Fructus.

关 键 词：诃子 鞣酸 提取物 红外光谱 

分 类 号：R284.1[医药卫生—中药学]