RP-HPLC-ELSD测定草乌中准噶尔乌头碱的含量  

Determination of Songorine in Aconiti Kusnezoffii Radix by RP-HPLC-ELSD

作　　者：彭劭[1] 刘莉萍[2] 金永日[1] 李绪文[1] 

机构地区：[1]吉林大学化学学院,长春130012 [2]深圳职业技术学院应用化学与生物技术学院,广东深圳518055

出　　处：《中国实验方剂学杂志》2016年第11期80-83,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:建立测定草乌中准噶尔乌头碱含量的方法。方法:采用反相高效液相色谱法,Agilent Extend-C18色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-0.1%三乙胺溶液(40∶60),流速1.0 m L·min^(-1),柱温25℃;蒸发光散射检测器(ELSD)的漂移管温度96℃,气体(空气)压力2.50 bar,撞击器呈关闭状态。结果:准噶尔乌头碱在1.5~30μg呈良好线性关系(r=0.999 5),平均回收率为96.86%,RSD 1.7%。结论:该方法简便、准确、快速,可用于测定草乌中准噶尔乌头碱的含量,为草乌的质量评价提供依据。Objective： To establish a method for determining the content of songorine（ a kind of diterpenoid alkaloids） in Aconiti Kusnezoffii Radix. Method： RP-HPLC was carried out on an Agilent Extend-C18（ 4. 6 mm × 250 mm,5 μm） with acetonitrile-0. 1% triethylamine solution（ 40∶ 60） as the mobile phase at a flow rate of 1. 0 m L·min^-1and column temperature of 25 ℃. The drift tube temperature of ELSD was set at 96 ℃,with the gas（ air） pressure of 2. 50 bar,and the impactor was in a closed status. Result： The songorine showed good linear relationship in the range of 1. 5-30 μg（ r = 0. 999 5）. Its average recovery rate was 96. 86% with RSD of 1. 7%. Conclusion： This established method is simple,quick and accurate,which can be used to determine the content of songorine in Aconiti Kusnezoffii Radix and provide reference for quality evaluation of Aconiti Kusnezoffii Radix.

关 键 词：草乌 准噶尔乌头碱 反相高效液相色谱 质量评价 

分 类 号：R284.1[医药卫生—中药学]