大鼠尿液中秦皮甲素和秦皮乙素代谢物的HPLC-MS^n分析  

Study on Metabolites of Aesculin and Aesculetin in Rat Urine by HPLC-MS^n

作　　者：杨建华[1,2] 王晓梅[2] 迪丽拜尔.马木提 杨秀娟[2] 胡君萍[2] 

机构地区：[1]新疆医科大学第一附属医院,乌鲁木齐830054 [2]新疆医科大学药学院,乌鲁木齐830011

出　　处：《中国实验方剂学杂志》2016年第11期93-96,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30960526;81560688)

摘　　要：目的:探索秦皮乙素和秦皮甲素在大鼠尿液中的代谢物,以期阐明这2种药物在体内作用的化学基础。方法:单次灌胃给药秦皮乙素和秦皮甲素后收集48 h内大鼠尿液,经固相萃取法处理,采用HPLC-MS^n检测代谢产物,色谱条件为流动相甲醇-0.2%甲酸(12∶88),流速1.0 m L·min^(-1),进样量5μL,柱温35℃,检测波长339 nm;质谱条件为电喷雾离子化方式,正离子扫描模式,喷雾电压3.0 k V,碰撞气流量0.16 m L·min^(-1),洗脱溶剂温度350℃,离子源温度450℃。结果:从大鼠尿液中发现并初步鉴定了5个代谢产物的化学结构,主要为秦皮乙素的水解产物、还原产物、甲基化产物及硫酸化产物。结论:推测秦皮甲素在体内先水解为秦皮乙素,再进行水解、还原、甲基化及硫酸化反应。Objective： To investigate the metabolites of aesculin and aesculetin in rat urine. Method：After single oral administration of aesculin and aesculetin to rats,urine was collected within 48 h and pretreated by solid-phase extraction method. Metabolites were determined by a HPLC-MS^n method. Result： Five major metabolites were found and their chemical structures were elucidated as hydrolysate, reduzate, methylated metabolite and sulfated product, respectively. Conclusion： According to the rule of metabolism, aesculin is hydrolyzed as aesculetin which were further metabolized in vivo. The method can be used to identify the structures of active components responsible for pharmacological effects.

关 键 词：秦皮甲素 秦皮乙素 代谢产物 HPLC-MSn 

分 类 号：R969.1[医药卫生—药理学] R945[医药卫生—药学]