隐丹参酮抑制恶性神经胶质瘤细胞C6增殖及对JAK2-STAT3信号通路的影响  

Cryptotanshinone inhibits proliferation of malignant glioma C6 cells and its influence on JAK2-STAT3 signal pathway

作　　者：唐焕焕 沈晓燕[2] 段小群[1] 徐庆[1] 苑博[1] 张嫦丽 吕良[1] 

机构地区：[1]桂林医学院药学院药理学教研室,桂林541001 [2]复旦大学药学院药理学与生物化学教研室,上海200120

出　　处：《安徽医科大学学报》2016年第6期769-772,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81460619);广西自然科学基金青年项目(编号:2015GXNSFBA139178)

摘　　要：目的评估隐丹参酮对恶性神经胶质瘤细胞C6的增殖作用及对JAK2-STAT3信号通路的影响。方法使用MTT法评价隐丹参酮对C6细胞存活率的影响,使用hochest染色和Brd U插入法检测隐丹参酮对C6细胞凋亡和增殖的影响,使用Western blot法检测隐丹参酮对C6细胞p-JAK2和p-STAT3(Tyr705)的影响。结果隐丹参酮显著抑制C6的生长和增殖(P<0.05),但不促进凋亡。隐丹参酮显著抑制p-STAT3(Tyr705)蛋白表达,但不影响p-JAK2。结论隐丹参酮是恶性神经胶质瘤C6的抗增殖剂,其作用机制与抑制STAT3信号有关。Objective To evaluate the effect of cryptotanshinone（ CTS） on the proliferation of malignant glioma C6 cells and its influence on JAK2-STAT3 signal pathway. Methods MTT was used to evaluate the survival rate of CTS on C6 cells. Hochest staining and Brd U assay was used to detect the cell apoptosis and proliferation. Western blot was used to detect the expressions of p-JAK2 and p-STAT3（ Tyr705）. Results CTS inhibited C6 cells growth and proliferation significantly（ P〈 0. 05）,but not induced apoptosis. CTS decreased phosphorylation of p-STAT3（ Tyr705）,but not p-JAK2. Conclusion CTS may be a potential anti-proliferation agent of malignant glioma C6 cells and that its mechanism may be related to the inhibition of STAT3 signaling.

关 键 词：恶性神经胶质瘤 增殖 JAK2-STAT3信号通路 

分 类 号：R966[医药卫生—药理学]