人参对胃癌SGC-7901侧群细胞裸鼠移植瘤生长及Bcl-2、Bax表达的影响  

Effect of Ginseng on human gastric cancer SGC-7901 side population cells xenograft tumor in nude mice and the expression of Bcl-2,Bax

作　　者：钱军[1] 刘元[1,2] 郭成旭 朱超[1] 承泽农[3] 

机构地区：[1]蚌埠医学院第一附属医院肿瘤外三科,蚌埠233000 [2]蚌埠医学院研究生部,蚌埠233000 [3]蚌埠医学院第一附属医院病理科,蚌埠233000

出　　处：《安徽医科大学学报》2016年第6期773-777,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金资助项目(编号:81173386)

摘　　要：目的探讨人参对胃癌SGC-7901侧群(SP)细胞裸鼠移植瘤生长及Bcl-2、Bax表达的影响。方法分选SP和非侧群(NSP)细胞;熬制人参汤剂,制备人参含药血清;Western blot法检测细胞中Bcl-2、Bax表达;建立裸鼠移植瘤模型,绘制瘤体生长曲线,计算抑瘤率;免疫组化法检测瘤体Bcl-2、Bax的表达。结果 SP细胞生理盐水(NS)组移植瘤体积明显大于NSP细胞NS组(P<0.05),NS组移植瘤体积明显大于相应人参组,差异有统计学意义(P<0.01),SP细胞人参组移植瘤体积与NSP细胞人参组差异无统计学意义;SP组和NSP组人参的抑瘤率分别为43.04%、31.55%;Western blot及免疫组化显示人参可抑制移植瘤Bcl-2、诱导Bax的表达,与相应NS组比较,差异有统计学意义(P<0.05,P<0.01)。结论 SP细胞的成瘤能力较NSP细胞强,人参可以抑制裸鼠移植瘤的生长,机制可能与诱导细胞凋亡相关。Objective To explore the affection of Ginseng on the growth of humangastric cancer SGC-7901 side population（ SP） cells xenografts in nude mice and expression of Bcl-2,Bax. Methods Sorting out SP cells and non-side population（ NSP） cells by flow cytometry; preparation of Ginseng decoction; tumor volume were measured to draw tumor growth curves and the tumor weight inhibition rates were calculated with tumor weight. The expressions of Bcl-2,Bax were detected by immunohistochemical technology,preparation containing Ginseng drug serum,Western blot technique was used to detect the expression of Bcl-2,Bax. Results The volume of transplanted tumors in SP cells NS group was bigger than group of NSP cells NS group（ P〈0. 05）. The tumor volume of NS was bigger than Ginseng group（ P〈0. 01）. The tumor inhibitory rates in the group of Ginseng in SP cells and NSP cells were 43. 04%,31. 55%,respectively. The results of Western blot and immunohistochemical staining showed that Ginseng could inhibit the expression of Bcl-2 and induce the expression of Bax,the difference was statistically significant（ P〈0. 05,P〈0. 01）. Conclusion The tumorigenicity of SP cells is stronger than NSP cells. Ginseng can restrain the growth of the nude mouse transplanted tumor,and the mechanism may be associated with inducing cell apoptosis.

关 键 词：侧群细胞 非侧群细胞 裸鼠移植瘤 人参 

分 类 号：R965.2[医药卫生—药理学]