雷公藤多苷干预舒尼替尼引起的小鼠肾足细胞凋亡及相关蛋白表达的机制研究  

Research on the mice podocyte apoptosis and expression of the associated protein caused by TWP intervene sunitinib

作　　者：江超[1] 陈映霞[1] 秦叔逵[1] 杨爱珍[2] 马兴群[1] 成远[1] 曹梦苒[1] 闻妹 

机构地区：[1]安徽医科大学解放军八一临床学院肿瘤内科,南京210002 [2]安徽医科大学解放军八一临床学院中心实验室,南京210002

出　　处：《安徽医科大学学报》2016年第6期800-804,共5页Acta Universitatis Medicinalis Anhui

基　　金：南京军区重点课题基金项目(编号:14ZD21)

摘　　要：目的观察舒尼替尼对体外培养小鼠肾足细胞的影响,并探讨雷公藤多苷(TWP)对舒尼替尼所致小鼠肾足细胞损伤的保护作用及其机制。方法体外培养小鼠肾足细胞,不同分组给药处理后,采用MTT法、流式细胞术、Western blot法检测小鼠肾足细胞的增殖抑制率、凋亡率及相关蛋白(Nephrin、CD2AP)的表达情况。结果小鼠肾足细胞增殖抑制率随着舒尼替尼浓度及作用时间的增加而增加(P<0.01);48 h凋亡率随舒尼替尼浓度增加而增加(P<0.01)。TWP可降低48 h舒尼替尼引起的小鼠肾足细胞增殖抑制率和凋亡率(P<0.05),同时可抑制舒尼替尼引起的Nephrin、CD2AP表达量降低(P<0.05)。结论舒尼替尼可损伤小鼠肾足细胞;而TWP通过上调Nephrin、CD2AP蛋白表达减轻舒尼替尼导致的足细胞损伤。Objective To observe the effect of sunitinib culturing mice podocyte in vitro,moreover,probe into the protective effect of Tripterbium wilfordii polyglycosidium（ TWP） to the cell trauma which caused by sunitinib and its mechanisms. Methods Cultured mice podocyte in vitro,after given medicine to different groups,detected the proliferation inhibiting rate,apoptosis rate and the expression status of the mice podocyte related protein（ Nephrin,CD2AP） by MTT,FCM and Western blot methods. Results The proliferation inhibition rate of the mice podocyte was increased with the increasing of sunitinib dose and treated time（ P 0. 01）. The apoptosis rate of mice podocyte increased with the increasing of the dose of sunitinib at 48 h（ P 〈0. 01）. TWP could decrease the proliferation inhibition and apoptosis of the mice podocyte which caused by sunitinib at 48 h（ P〈 0. 05）,at the same time it could inhibit the decrease of the mice podocyte related protein（ Nephrin,CD2AP） expression which caused by sunitinib（ P〈 0. 05）. Conclusion Sunitinib can damnify mice podocyte,but TWP can induce the mice podocyte cell trauma caused by sunitinib through up-regulating Nephrin and CD2 AP expression.

关 键 词：舒尼替尼 抗血管生成 雷公藤多苷 小鼠肾足细胞 蛋白尿 

分 类 号：R979.1[医药卫生—药品]