丙泊酚对原代培养大鼠皮层神经元BDNF和p75NTR水平的影响  

Effects of propofol treatment on BDNF and p75NTR expression in primary cultured cortical neurons

作　　者：李建立[1] 崔红赏[2] 王蓓[3] 吴红海[4] 侯艳宁[4] 

机构地区：[1]河北省人民医院麻醉科,石家庄050051 [2]河北省人民医院胸外科,石家庄050051 [3]河北省人民医院妇产科,石家庄050051 [4]白求恩国际和平医院药剂科,石家庄050082

出　　处：《安徽医科大学学报》2016年第6期818-821,共4页Acta Universitatis Medicinalis Anhui

基　　金：河北省卫生厅指令课题(编号:ZL20140095)

摘　　要：目的探讨丙泊酚对原代培养大鼠皮层神经元凋亡的影响及可能机制。方法原代培养7 d的大鼠皮层神经元,随机分为两组:溶剂对照组(给予相同容积的20%脂肪乳剂),丙泊酚组(丙泊酚终浓度为500μmol/L),上述药物处理皮层神经元12 h后,用光学显微镜观察两组皮层神经元的形态学变化,MTT法检测神经元存活率的变化,Western blot法检测神经元脑源性神经营养因子(BDNF)、p75神经营养因子受体(p75NTR)以及B淋巴细胞瘤-2基因(Bcl-2)蛋白水平的变化。结果与溶剂对照组比较,丙泊酚组光镜下观察显示皮层神经元数量明显减少,胞体立体感消失,细胞轮廓不清,神经元轴突断裂。神经元存活率显著性下降(P<0.01),BDNF和Bcl-2蛋白水平显著性下降(P<0.01),p75NTR蛋白水平显著性增加(P<0.01)。结论丙泊酚可引起发育期原代培养皮层神经元损伤,其机制可能与下调BDNF、Bcl-2和上调p75NTR蛋白水平有关。Objective To investigate the effect of propofol exposure on neuroapoptosis in primary cultured cortical neurons and the mechanisms. Methods Cortical neurons were primarily cultured for seven days,then divided into two groups： vehicle-control group（ treated with equal valume of 20% intralipid）,propofol-treated group（ treated with 500 μmol / L propofol）. The neurons were treated for 12 h. The structure of neurons was analyzed using microscope,the neuron viability was determined by MTT,and Western blot was performed to detect the levels of BDNF,p75 NTR and Bcl-2. Results Lack of three-dimensional sense,faded color,unclear outline were observed,and fractured neuron axons or neurons death were observed in neurons treated by 500 μmol / L propofol. Compared with vehicle-control group,the neuron viability decreased greatly（ P 〈0. 01）,BDNF and Bcl-2 levels decreased greatly（ P 〈0. 01） and p75 NTR level increased greatly in propofol treatment group（ P〈 0. 01）. Conclusion Propofol induces neuroapotosis in primary cultured cortical neurons,which is associated with the decreased levels of BDNF and Bcl-2 and the increased level of p75 NTR.

关 键 词：原代培养皮层神经元 丙泊酚 神经凋亡 BDNF P75NTR BCL-2 

分 类 号：R971.2[医药卫生—药品] R965[医药卫生—药学]