亚洲棉bZIP蛋白家族的鉴定及GaFD基因的组织表达分析  

Identification of bZIP Protein Family in Gossypium arboreum and Tissue Expression Analysis of GaFDs Genes

作　　者：张彦楠 蔡大润[1] 黄先忠[1] 

机构地区：[1]石河子大学生命科学学院/植物基因组学实验室,新疆石河子832003

出　　处：《作物学报》2016年第6期832-843,共12页Acta Agronomica Sinica

基　　金：教育部新世纪优秀人才支持计划项目(NCET-12-1072);国家自然科学基金项目(31360366);新疆生产建设兵团博士基金项目(2012BB007)资助~~

摘　　要：碱性亮氨酸拉链(basic leucine zipper,bZIP)是真核生物中数量最多并且最具多样性的转录因子之一,参与植物生长发育及响应生物和非生物胁迫。本研究利用亚洲棉(Gossypium arboreum)全基因组数据库,通过生物信息学分析分布在13条染色体上的159个bZIP家族基因的全序列。系统进化、基因结构和保守基序分析表明这些基因分成13个亚家族。其中,A亚家族有3个GaFD基因GaFD1、GaFD2和GaFD3,通过实时荧光定量PCR分析3个GaFD基因在不同组织中的表达,结果表明GaFD1和GaFD2在SAM中的表达量最高,GaFD3在茎中表达量最高。研究表明棉花基因组中具有数量众多的bZIP家族成员,不同基因结构及FD基因不同的表达特征表明bZIP基因在棉花生长发育中可能具有不同的功能,这些结果为进一步解析棉花bZIP家族基因的功能和作用机理积累了有价值的资料。The basic leucine zipper（bZIP） is one of the largest and most diverse transcription factors in eukaryotes, and is involved in various processes of plant growth and development and in response to biotic and abiotic stresses. In this study, 159 bZIP family genes were identified and their complete gene sequences were obtained by using bioinformatics analysis method, based on Gossypium arboreum whole genome database, and 159 bZIP genes were loaded on 13 chromosomes. The 159 bZIP genes were categorized into 13 groups based on their phylogenetic relationships, gene structures and conserved motifs. In addition, three GaFD homologous genes GaFD1, GaFD2, and GaFD3 were identified, which belongs to A subfamily in G. arboretum. The expression patterns of GaFD genes in different tissues were determined by using quantitative Real-time reverse transcription PCR（qRT-PCR） method. The results showed that GaFD1 and Gh FD2 were preferentially expressed in the shoot apical meristem（SAM）, whereas GaFD3 was preferentially expressed in stem. The results revealed that a number of bZIP family members exist in cotton genome, and FD genes with different structures and expression patterns play different roles in the development of cotton, which provides valuable information for dissecting the function and mechanism of bZIPs in cotton.

关 键 词：碱性亮氨酸拉链 亚洲棉 FD 进化分析 基因表达 

分 类 号：S562[农业科学—作物学]