1株甘蔗根际促生细菌的鉴定及其生物学功能研究初报  

Identification and Determination of Biological Functions of Plant Growth Promoting Rhizobacteria of Sugarcane

作　　者：史国英[1,2] 魏源文[2] 林丽[3] 杨丽涛[3,4] 李杨瑞[3,4] 胡春锦[1,2] 

机构地区：[1]广西农业科学院微生物研究所,广西南宁530007 [2]广西作物遗传改良生物技术重点开放实验室,广西南宁530007 [3]中国农业科学院甘蔗研究中心,广西南宁530007 [4]广西大学,广西南宁530005

出　　处：《西南农业学报》2016年第5期1110-1114,共5页Southwest China Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(31101122;31171504);广西农业科学院基金项目(2013YT04;2013JQ09;2014GW07);"广西特聘专家"专项

摘　　要：为了给甘蔗根际促生细菌的开发和利用提供理论依据,以及对微生物功能基因的挖掘利用提供有价值的菌种资源。利用常规分离方法从甘蔗根际中分离获得菌株GG08160,通过固氮酶活性测定、固氮酶基因nif H扩增及序列分析鉴定该细菌的固氮能力;采用Salkowski比色法和溶磷圈D/d比值方法分别测定该菌株分泌IAA以及对无机磷的降解能力,利用含有浓度梯度草甘膦的培养基胁迫培养测定该菌株对草甘膦的抗性;采用盆栽接种法明确菌株对甘蔗幼苗的实际促生长作用;根据形态学、Biolog检测和16S rRNA序列分析对菌株GG08160进行鉴定,确定该菌株的分类地位。研究结果表明,菌株GG08160具有固氮、分泌IAA和降解无机磷的功能,能显著促进甘蔗幼苗的生长;该菌株在草甘膦含量达250 m M的培养基质中仍能正常生长,具有高抗草甘膦的活性;根据菌株培养性状、Biolog检测和16S r DNA序列分析结果,把该菌株鉴定为克雷伯氏菌属(Klebsiella sp.)细菌。The aim of this work was to screen out and identify a valuable resource for the study of biological fertilizer,and provide evidences for the exploration and utilization of associative nitro-gen fixation bacteria in rhizosphere of sugarcane. This study exploited the conventional methods of separation of rhizosphere bacteria GG08160 from sugarcane,and the strain was found to have N2 fixing ability as demonstrated by the acetylene reduction assay and nif H gene amplification and sequence analysis,and biological functions of the strain including IAA secretion,phosphate solubilization,glyphosate-resistance were studied by Salkowski colorimetry,phosphorus hole（D / d ratio） and by gradient dilution glyphosate selected culture. Identification of the strain species according to the microscopic observation and 16 S r DNA sequence analysis results. A high glyphosate-res-istance bacterium strain GG08160 was isolated from rhizosphere of sugarcane,which can grow well in glyphosate solution with the concentration of 250 m M. Also,the strain possessed the capacity of nitrogen fixation,IAA secretion and phosphate solubilization. Inoculation test showed that the train GG08160 can remarkably promote the growth of sugarcane plantlets. Based on phenotypic characteristics,biolog system test,and 16 S rRNA sequence analysis,the strain GG08160 was identified as Klebsiella sp..

关 键 词：甘蔗 根际促生细菌 分类鉴定 生物学功能 

分 类 号：S566.1[农业科学—作物学]