模拟载人飞船内失重和噪声环境对大鼠肺组织损伤及caspase-3表达的影响  

Effect of simulated microgravity and noise on lung injury and the expression of caspase-3 in rats in spaceship

作　　者：陈娜[1] 彭文鸿[2] 吴玮[1] 司少艳[3] 韩浩伦[1] 张建中[4] 吴继华[4] 冯凯[3] 丁瑞英[1] 

机构地区：[1]解放军306医院耳鼻咽喉头颈外科,北京100101 [2]解放军306医院呼吸内科,北京100101 [3]解放军306医院特种医学实验研究中心,北京100101 [4]解放军306医院病理科,北京100101

出　　处：《临床肺科杂志》2016年第7期1165-1167,1186,共4页Journal of Clinical Pulmonary Medicine

基　　金：全军十二五重大课题子课题(No AWS11J003)

摘　　要：目的研究模拟载人飞船内失重和噪声环境对大鼠肺组织病理及细胞凋亡的影响。方法 30只SD大鼠随机分为两组:空白组(6只)和实验组(24只)。实验组给予持续尾部悬吊模拟飞船舱内失重环境及复合噪声暴露,分别于暴露1周、2周、4周后处死动物,即刻取肺组织,观察大体标本及HE染色、免疫组化染色观察caspase-3在肺脏的表达。空白组不做任何处理,常规饲养后行以上检查。结果所有实验组大鼠肺水肿,肺泡隔肿胀,上皮细胞破坏。免疫组化结果显示:实验组大鼠肺泡间质、支气管上皮细胞及血管内皮细胞中caspase-3的表达较空白组明显增强,且在一定时间内随暴露时间的推移表达有逐渐增强的趋势。结论模拟载人飞船内失重和噪声环境对大鼠肺组织有明显的损伤作用,该损伤可能与肺细胞的凋亡有关。Objective To investigate the effect of simulated microgravity and noise in spaceflight on lung injury and apoptosis in rats. Methods 30 rats were randomly divided into the control group （ n = 6） and the experi- mental group （n = 24）. The experimental group were exposed to -30° head down tilt as simulated microgravity and noise including steady-state noise and impulse noise of spaceship environment. The control group was kept in normal conditions without exposure to microgravity and noise. After 1,2, and 4 weeks＇exposure to microgravity and noise respectively, the rats＇lungs tissue were tested by HE staining, and the expression of caspase-3 in lung tissue cells were evaluated by immunohistochemical staining. Results The rats exposed to microgravity and noise showed lung edema and inflammatory cell infiltration. The damage has the same tendency with cell apoptosis condition. Compared with the control group, Caspase-3 expression in the experimental group was positive in lung cells. The expression of caspase-3 had a growing tendency with the exposure time. Conclusion The environment of simulated microgravity and inboard noise of spaceship leads to obvious lung injury which may related to apoptosis of lung cell.

关 键 词：载人飞船 模拟失重 噪声 细胞凋亡 Caspase-3 

分 类 号：R852.22[医药卫生—航空、航天与航海医学]