乌腺金丝桃总黄酮对大鼠心肌细胞心律失常模型L型钙通道电流的影响  

Influence of total flavonoids from Hypericum attenuatum Choisy on L-type calcium channel currents of cardiomyocytes in arrhythmic model rats

作　　者：马育轩[1] 王艳丽 高彦宇[1] 胡晓阳[1] 李冀[1] 

机构地区：[1]黑龙江中医药大学,哈尔滨150040 [2]黑龙江护理高等专科学校,哈尔滨150086

出　　处：《中华中医药杂志》2016年第6期2119-2122,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81373536);黑龙江中医药大学优秀领军人才支持计划(No.2012);黑龙江中医药大学校科研基金资助面上项目(No.201501)~~

摘　　要：目的:探讨乌腺金丝桃总黄酮对大鼠心肌细胞心律失常模型L型钙通道电流(I_(Ca,L))的影响。方法:应用全细胞膜片钳技术观察乌腺金丝桃总黄酮对大鼠心肌细胞心律失常模型I_(Ca,L)电流密度峰值、I_(Ca,L)I-V曲线、I_(Ca,L)稳态激活曲线、I_(Ca,L)稳态失活曲线及I_(Ca,L)失活后恢复曲线中时间常数t值的影响,探讨其对模型细胞I_(Ca,L)异常的恢复作用。结果:乌腺金丝桃总黄酮对模型细胞不同去极化水平下I_(Ca,L)电流密度峰值有抑制作用,使模型细胞I_(Ca,L)I-V曲线明显上移,I_(Ca,L)稳态激活曲线和稳态失活曲线向去极化方向移动,恢复乌头碱减小的I_(Ca,L)失活后恢复曲线中t值,延长其恢复时间。结论:乌腺金丝桃总黄酮对大鼠心肌细胞心律失常模型I_(Ca,L)异常有较好的恢复作用,其抗心律失常的机制可能与抑制I_(Ca,L)有关。Objective： To observe the effects of total flavonoids from Hypericum attenuatum Choisy on L-type calcium channel currents（I_（Ca, L）） of cardiomyocytes in arrhythmic rats induced by aconitine. Methods： Whole cell patch clamp technique was used to observe the effects of total flavonoids from Hypericum attenuatum Choisy on I_（Ca, L） of the model cells about the peak current density, the curve of I-V, the steady-state activation curve, the steady-state inactivation curve and time constant t value of recovery curve after inactivation to discuss the recovery action on exceptional IK1. Results： Total flavonoids from Hypericum attenuatum Choisy had a significant inhibitory effect on I_（Ca, L） peak current density on different level of depolarization on the model cells. Total flavonoids from Hypericum attenuatum Choisy made the I_（Ca, L） I-V curve moved up, made the I_（Ca, L） steadystate activation curve and the I_（Ca, L） steady-state inactivation curve moved towards the direction of depolarization and prolong the recovery time of the I_（Ca, L）. Conclusions： The experimental results point out that total flavonoids from Hypericum attenuatum Choisy has a good healing effect on I_（Ca, L） of arrhythmic cardiomyocytes in rats and its antiarrhythmic mechanism may be related to the inhibition of L-type calcium channel current.

关 键 词：心肌细胞心律失常模型 乌腺金丝桃总黄酮 L型钙通道电流 

分 类 号：R285.5[医药卫生—中药学]