芎芪颗粒对肾间质纤维化大鼠肾组织HGF、MMP-3、TIMP-1的影响  

Effects of Xiongqi Granules on HGF, MMP-3 and TIMP-1 in kidney of rats with renal interstitial fibrosis

作　　者：杨璋斌[1] 李安[1] 杨璇[1] 师晶丽[1] 

机构地区：[1]中山大学附属第五医院,珠海519000

出　　处：《中华中医药杂志》2016年第6期2142-2145,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：广东省中医药局资金项目(No.20121144)~~

摘　　要：目的:探讨芎芪颗粒抗肾间质纤维化的作用机制。方法:将32只雄性Wistar大鼠随机分入假手术组、UUO模型组、蒙诺治疗组和芎芪颗粒治疗组,采用相应处理后取肾脏组织,以Western Blot检测HGF蛋白表达,以免疫组织化学法检测MMP-3和TIMP-1。结果:与假手术组比较,UUO模型组的肾脏组织HGF蛋白相对表达量下降(P<0.01),MMP-3和TIMP-1均升高(P<0.01)。与UUO模型组比较,蒙诺治疗组和芎芪颗粒治疗组的肾脏组织HGF蛋白相对表达量和MMP-3均升高(P<0.01),TIMP-1均下降(P<0.01)。结论:芎芪颗粒可能通过提高HGF的表达及诱增其下游因子MMP-3的表达,抑制TIMP-1的表达,恢复MMP-3/TIMP-1的失衡,来达到抗肾间质纤维化的作用。Objective： To explore the mechanism underlying Xiongqi Granules prevented renal interstitial fibrosis. Methods： Thirty-two male Wistar rats were randomly divided into the sham-operation group, unilateral ureteral obstruction（UUO） group, monopril group and Xiongqi Granules group. The protein expression of hepatocyte growth factor（HGF） was detected with Western Blot, and marix metalloproteinase-3（MMP-3） and tissue inhibitor of metalloproteinase-1（TIMP-1） were detected with immunohistochemical method. Results： Compared with the sham-operation group, the protein expression of HGF in the UUO group was lower（P〈0.01）, and the expression of MMP-3 and TIMP-1 were higher（P〈0.01）. Compared with the UUO group, the protein expression of HGF and MMP-3 in the monopril and Xiongqi Granules group were higher（P〈0.01）, while the expression of TIMP-1 was lower（P〈0.01）. Conclusion： Xiongqi Granules may achieve the effect of anti-renal interstitial fibrosis by improving the expression of HGF and its downstream factor MMP-3, as well as inhibiting the expression of TIMP-1, ultimately recovering the imbalance of MMP-3/TIMP-1.

关 键 词：芎芪颗粒 肾间质纤维化 肝细胞生长因子 基质金属蛋白酶-3 金属蛋白酶组织抑制物-1 

分 类 号：R285.5[医药卫生—中药学]